Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NSS96_RS05130 Genome accession   NZ_CP151980
Coordinates   1016337..1020041 (+) Length   1234 a.a.
NCBI ID   WP_305933608.1    Uniprot ID   -
Organism   Bacillus sp. FSL W7-1336     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1011337..1025041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS96_RS05115 (NSS96_05115) - 1011643..1012053 (+) 411 WP_098676793.1 DoxX family protein -
  NSS96_RS05120 (NSS96_05120) - 1012251..1012697 (-) 447 WP_061109640.1 ferritin-like domain-containing protein -
  NSS96_RS05125 (NSS96_05125) addB 1012850..1016359 (+) 3510 WP_098676794.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NSS96_RS05130 (NSS96_05130) addA 1016337..1020041 (+) 3705 WP_305933608.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NSS96_RS05135 (NSS96_05135) sbcD 1020068..1021243 (+) 1176 WP_024426531.1 exonuclease subunit SbcD -
  NSS96_RS05140 (NSS96_05140) - 1021240..1024635 (+) 3396 WP_305933609.1 SMC family ATPase -
  NSS96_RS05145 (NSS96_05145) - 1024656..1024955 (+) 300 WP_034282144.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142438.62 Da        Isoelectric Point: 4.9696

>NTDB_id=983171 NSS96_RS05130 WP_305933608.1 1016337..1020041(+) (addA) [Bacillus sp. FSL W7-1336]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQINELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILRLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIDYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLLPDFERYQSKTYLDFIGPALIRHQAMSSILEETGDVVLSHPSAFTISFTQASDL
LKEDMSLEKKQQDEVVQALMEGLPVEEYGDADEKVAERLSWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPSSLRKAR
ATMYDRPAFMKKKTLTAAEQGTAMHTVMQHIALPSDEPFDEIRIHQLLDFLQQRDLLTNEQVQSIDQEGIAAFFSTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLEAAVPILKKRYE
TQIALYAKAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=983171 NSS96_RS05130 WP_305933608.1 1016337..1020041(+) (addA) [Bacillus sp. FSL W7-1336]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATCCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCTAAGAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
CACCCTGCACTCCTTTTGTTTGCAGGTTTTGCGCACCTTTTACTATGAGATTGACCTTGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGTTACACTTCTGACCGTCATGATTTAGATTTGCAATGGCTTGTGAAGAGAATTTATGAGTT
TTCCAGATCTCATCCTTCACCTGAACAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGACCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTCGAACGAGCT
CTCGCCCTATCAAAAGAACCGGGTGGCCCCGCGCCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAGATCAATGAATT
AATCAGGCACCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTCAAA
GATGAATACTTTATGCGCAGTCCCGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCGGTGGTCGAAACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAGAGCGAAGCAAGAAAAATCAATCGTGGATTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGACGCGGAAGGAAATTTGATTGAAACAGAAGCGGCCAAGTACTATCAACAGCAG
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAGGAAACGATTTTAAGGCTTGTATCTAAAGGGGA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGTTTTAGACTGGCTGAGCCTATGC
TCTTTTTAAACAAATATAAACAATTTACACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGATTACGA
TGAACAAGCTGAATTAAAGCTTGGAGCTAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTACATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAGCGAGAAGAACTAGAGACCGTACAACTAGAAGCGAGAGTCATTGCTAAAAAA
ATAAGAGAACTGGTGGAGCAGCCATTTCAAGTGTACGATGCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTACTTCGCTCCATGCCTTGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCGGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTCATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTATTAAGATCACCAATTGTTCATTTAGACGAAAATGAAATGGCACTGATTCGCACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGGTTTTTCAGGTTGCTGCGTAAGTGGCGTGATTTCTCCATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTTGGCGGGATGCCTGGAGGCAAGCAGAGACAGGCGAACCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCCTTTAGAGGGCTGTTCCGTTTTCTTCGTTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGTGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCCTACTTGCTTGA
TAAAGAATTAGGCTTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGCTATGCAACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGGAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTAGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTTGTCGGCTCTGTCAAAAATCAGGTGAAGGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAGGACTGGCTCCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGCCCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGATGTTGTTCTTTCACACCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAGGACGAGGTGGTACAAGCTTTGATGGAAGGTCTGCCTGTTGAAGA
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCATACCTAGCGGCCTCACAAGTCGGCACAA
AGCAGTCAGTTTCCGAGATAAAAAGAATGAAAGAAATACAGGATGAGTATAGTGTTCCTTCTTCTCTTCGAAAAGCCCGT
GCCACGATGTATGACCGGCCGGCTTTCATGAAGAAAAAAACACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
CATGCAGCATATTGCGCTTCCTTCTGACGAGCCCTTCGATGAAATCCGTATACATCAGCTGCTCGATTTCTTACAGCAGC
GAGACTTGCTAACAAATGAACAAGTTCAGTCAATTGATCAAGAAGGAATTGCAGCTTTTTTCTCTACTTCTATTGGTCAA
AAGCTGCTGAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTTATTCAAGGGATGATTGACTGTTTATTTGAAACGGATGGAAAACTATATTTACTAG
ATTATAAAACAGATAGAGTGCAAGGCAGATATACAGGCGGTCTTGAAGCAGCTGTACCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTATATGCAAAAGCAGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-462)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.424

100

0.665

Multiple sequence alignment