Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   NST32_RS12065 Genome accession   NZ_CP151978
Coordinates   2328481..2329251 (-) Length   256 a.a.
NCBI ID   WP_034280128.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0215     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2323481..2334251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST32_RS12045 (NST32_12045) - 2323574..2324395 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  NST32_RS12050 (NST32_12050) - 2324421..2325059 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  NST32_RS12055 (NST32_12055) glyS 2325227..2327272 (-) 2046 WP_034280130.1 glycine--tRNA ligase subunit beta -
  NST32_RS12060 (NST32_12060) glyQ 2327265..2328152 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  NST32_RS12065 (NST32_12065) recO 2328481..2329251 (-) 771 WP_034280128.1 DNA repair protein RecO Machinery gene
  NST32_RS12070 (NST32_12070) - 2329287..2329430 (-) 144 WP_003217288.1 YqzL family protein -
  NST32_RS12075 (NST32_12075) era 2329579..2330484 (-) 906 WP_007501305.1 GTPase Era -
  NST32_RS12080 (NST32_12080) - 2330477..2330875 (-) 399 WP_008356263.1 cytidine deaminase -
  NST32_RS12085 (NST32_12085) - 2330989..2331375 (-) 387 WP_024423020.1 diacylglycerol kinase family protein -
  NST32_RS12090 (NST32_12090) ybeY 2331353..2331832 (-) 480 WP_041106915.1 rRNA maturation RNase YbeY -
  NST32_RS12095 (NST32_12095) - 2331833..2333920 (-) 2088 WP_186318661.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29249.92 Da        Isoelectric Point: 8.4348

>NTDB_id=983097 NST32_RS12065 WP_034280128.1 2328481..2329251(-) (recO) [Bacillus sp. FSL L8-0215]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRA
IREDLFLTAYAAYMTELMDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKQQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=983097 NST32_RS12065 WP_034280128.1 2328481..2329251(-) (recO) [Bacillus sp. FSL L8-0215]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTCATGGCAAGAGGCGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGGCC
ATTCGGGAAGACTTATTTCTGACCGCATATGCCGCATATATGACGGAATTGATGGATAAAGGGACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAACGAAGGTACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTTGATCAATGTGTGCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCAATAAGAGATAACGGCTTCATTTGCCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCCGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTTGATGTGAAACAGCAGA
CGAAACAAGAAATTCGTCAAGTGCTTGATCACTACTATGACGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754

Multiple sequence alignment