Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST32_RS09145 Genome accession   NZ_CP151978
Coordinates   1784666..1785094 (+) Length   142 a.a.
NCBI ID   WP_034283541.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0215     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1779666..1790094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST32_RS09135 (NST32_09135) - 1780209..1783352 (+) 3144 WP_267542752.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  NST32_RS09140 (NST32_09140) - 1783546..1784454 (+) 909 WP_024425124.1 trypsin-like serine protease -
  NST32_RS09145 (NST32_09145) nucA/comI 1784666..1785094 (+) 429 WP_034283541.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST32_RS09150 (NST32_09150) - 1785240..1786088 (+) 849 WP_061109911.1 STAS domain-containing protein -
  NST32_RS09155 (NST32_09155) - 1786121..1786663 (-) 543 WP_061109912.1 IseA DL-endopeptidase inhibitor family protein -
  NST32_RS09160 (NST32_09160) - 1786959..1787408 (+) 450 WP_041109226.1 hypothetical protein -
  NST32_RS09165 (NST32_09165) lexA 1787454..1788074 (-) 621 WP_034283422.1 transcriptional repressor LexA -
  NST32_RS09170 (NST32_09170) yneA 1788233..1788544 (+) 312 WP_024425130.1 cell division suppressor protein YneA -
  NST32_RS09175 (NST32_09175) - 1788562..1789209 (+) 648 WP_034283427.1 recombinase family protein -
  NST32_RS09180 (NST32_09180) - 1789279..1789509 (+) 231 WP_024425132.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15702.90 Da        Isoelectric Point: 9.5681

>NTDB_id=983090 NST32_RS09145 WP_034283541.1 1784666..1785094(+) (nucA/comI) [Bacillus sp. FSL L8-0215]
MKRGWIAAVTLCFVIWMGTLLGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQES
LKGIPTKPGFDRDEWPMAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISRFPDGKRILFIVK

Nucleotide


Download         Length: 429 bp        

>NTDB_id=983090 NST32_RS09145 WP_034283541.1 1784666..1785094(+) (nucA/comI) [Bacillus sp. FSL L8-0215]
ATGAAAAGAGGCTGGATCGCTGCTGTCACCTTATGTTTTGTGATATGGATGGGCACGTTGTTAGGTGGATTTGGAGAGAA
GCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCCAAAGGAAAGGTACCCTGAAACAGGCAGTCATA
TTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACCGAAATGGAGCAGATGCCCGCAGGCAAGAATCA
TTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCAATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGC
AAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGAGCAGGCTCATGGGTCGGACACCAAATCAGCAGGTTTCCTGATG
GGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

83.803

0.486

Multiple sequence alignment