Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   NST32_RS08140 Genome accession   NZ_CP151978
Coordinates   1572304..1573206 (+) Length   300 a.a.
NCBI ID   WP_025092576.1    Uniprot ID   A0A5C0WNT3
Organism   Bacillus sp. FSL L8-0215     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1567304..1578206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST32_RS08120 (NST32_08120) - 1567967..1569706 (+) 1740 WP_340900370.1 glycosyltransferase -
  NST32_RS08125 (NST32_08125) - 1569703..1569978 (+) 276 WP_003211418.1 FlhB-like flagellar biosynthesis protein -
  NST32_RS08130 (NST32_08130) sucC 1570155..1571315 (+) 1161 WP_024426972.1 ADP-forming succinate--CoA ligase subunit beta -
  NST32_RS08135 (NST32_08135) sucD 1571338..1572240 (+) 903 WP_007499624.1 succinate--CoA ligase subunit alpha -
  NST32_RS08140 (NST32_08140) dprA 1572304..1573206 (+) 903 WP_025092576.1 DNA-processing protein DprA Machinery gene
  NST32_RS08145 (NST32_08145) topA 1573400..1575475 (+) 2076 WP_041108973.1 type I DNA topoisomerase -
  NST32_RS08150 (NST32_08150) trmFO 1575546..1576850 (+) 1305 WP_024424183.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  NST32_RS08155 (NST32_08155) xerC 1576913..1577830 (+) 918 WP_034281771.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34070.43 Da        Isoelectric Point: 8.8768

>NTDB_id=983086 NST32_RS08140 WP_025092576.1 1572304..1573206(+) (dprA) [Bacillus sp. FSL L8-0215]
MYNVSERMIFHRLKGLISPSLLTKWWKVDPELYINEETHHFKQDRALQTIDFTRLKQAEENEFPIFQHIVQAYLKQNIHM
IPITSPLYPSTLKNIYDPPPVLFLKGNVSYLNEEKSLGVVGTRVPSSYGEACVKKIVGELVKEEWMIVSGLAKGIDGLAH
KECIRKKGKTIGIIAGGFQHVYPKEHAQMAQYMGEHHLLLSEHPPYVKPEKWHFPMRNRLISALTKGTIVIQCKEKSGSL
ITAYQALEQGKEVFAVAGSIFDSNSTGPARLIQQGAKLVHSTKDILEEFSFHSVQYTELS

Nucleotide


Download         Length: 903 bp        

>NTDB_id=983086 NST32_RS08140 WP_025092576.1 1572304..1573206(+) (dprA) [Bacillus sp. FSL L8-0215]
ATGTACAACGTGTCCGAAAGAATGATTTTTCACCGCTTAAAAGGCCTCATCTCACCCTCATTATTAACAAAATGGTGGAA
AGTCGATCCTGAACTATATATAAATGAAGAAACACATCATTTTAAACAGGATCGAGCATTACAAACGATCGACTTTACCC
GCTTAAAACAAGCTGAAGAAAATGAATTCCCCATTTTTCAACACATCGTTCAAGCCTATTTAAAGCAAAACATTCACATG
ATTCCCATCACATCACCCTTATATCCCAGCACACTAAAAAATATTTATGATCCTCCCCCTGTGTTATTCCTAAAAGGAAA
CGTATCATATTTAAATGAAGAAAAAAGTTTAGGTGTAGTCGGCACGCGAGTTCCATCGTCTTATGGAGAAGCATGTGTGA
AGAAAATTGTTGGTGAGCTTGTCAAGGAAGAGTGGATGATTGTCAGTGGTTTAGCTAAAGGAATCGATGGACTGGCACAT
AAAGAGTGCATCAGGAAAAAAGGGAAAACAATCGGCATTATAGCAGGCGGCTTTCAGCATGTATACCCAAAAGAACATGC
ACAAATGGCTCAATACATGGGCGAACATCATTTGCTTTTGTCAGAGCACCCGCCTTATGTCAAACCAGAAAAGTGGCATT
TTCCAATGAGAAATCGTTTAATTAGTGCATTGACGAAAGGGACCATCGTGATTCAGTGCAAAGAAAAGAGCGGTTCTCTT
ATTACAGCCTATCAAGCACTTGAGCAAGGCAAAGAAGTATTTGCGGTTGCCGGATCCATCTTTGATTCTAATTCCACAGG
TCCGGCAAGACTCATTCAGCAGGGGGCAAAGCTTGTTCATTCCACAAAGGATATTTTAGAGGAATTCTCCTTCCACAGCG
TTCAATATACTGAACTCTCATAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WNT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

54.355

95.667

0.52
  dprA Legionella pneumophila strain ERS1305867

37.705

100

0.383

Multiple sequence alignment