Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NST32_RS01915 Genome accession   NZ_CP151978
Coordinates   356769..357197 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus sp. FSL L8-0215     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 351769..362197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST32_RS01885 (NST32_01885) - 351931..352260 (+) 330 WP_024424311.1 YckD family protein -
  NST32_RS01890 (NST32_01890) - 352304..353068 (-) 765 WP_217008882.1 MBL fold metallo-hydrolase -
  NST32_RS01895 (NST32_01895) - 353184..353600 (+) 417 WP_180951418.1 Lrp/AsnC family transcriptional regulator -
  NST32_RS01900 (NST32_01900) - 353629..354435 (+) 807 WP_217008883.1 AAC(3) family N-acetyltransferase -
  NST32_RS01905 (NST32_01905) - 354437..354823 (-) 387 WP_046313809.1 competence protein ComJ -
  NST32_RS01910 (NST32_01910) - 354944..356641 (-) 1698 WP_046313811.1 methyl-accepting chemotaxis protein -
  NST32_RS01915 (NST32_01915) nucA/comI 356769..357197 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  NST32_RS01920 (NST32_01920) - 357565..359634 (+) 2070 WP_098677313.1 hydantoinase/oxoprolinase family protein -
  NST32_RS01925 (NST32_01925) - 359624..361612 (+) 1989 WP_217008884.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=983065 NST32_RS01915 WP_024424305.1 356769..357197(-) (nucA/comI) [Bacillus sp. FSL L8-0215]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=983065 NST32_RS01915 WP_024424305.1 356769..357197(-) (nucA/comI) [Bacillus sp. FSL L8-0215]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCGTCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCGATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCAGACG
GCACAAGAATCCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535

Multiple sequence alignment