Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NST70_RS09295 Genome accession   NZ_CP151971
Coordinates   1958309..1959433 (-) Length   374 a.a.
NCBI ID   WP_061577075.1    Uniprot ID   -
Organism   Weizmannia sp. FSL K6-0777     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1953309..1964433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST70_RS09250 (NST70_09250) - 1953877..1954668 (+) 792 WP_014098030.1 YqhG family protein -
  NST70_RS09255 (NST70_09255) - 1954701..1954877 (-) 177 WP_014098029.1 YqzE family protein -
  NST70_RS09260 (NST70_09260) - 1954864..1955433 (-) 570 WP_237680863.1 shikimate kinase -
  NST70_RS09265 (NST70_09265) comGG 1955418..1955786 (-) 369 WP_014098027.1 competence type IV pilus minor pilin ComGG -
  NST70_RS09270 (NST70_09270) comGF 1955783..1956268 (-) 486 WP_035184588.1 competence type IV pilus minor pilin ComGF -
  NST70_RS09275 (NST70_09275) - 1956231..1956539 (-) 309 WP_035187947.1 type II secretion system protein -
  NST70_RS09280 (NST70_09280) comGD 1956526..1956969 (-) 444 WP_035187944.1 competence type IV pilus minor pilin ComGD -
  NST70_RS09285 (NST70_09285) comGC 1956966..1957277 (-) 312 WP_013859645.1 competence type IV pilus major pilin ComGC -
  NST70_RS09290 (NST70_09290) comGB 1957345..1958409 (-) 1065 WP_235601868.1 competence type IV pilus assembly protein ComGB -
  NST70_RS09295 (NST70_09295) comGA 1958309..1959433 (-) 1125 WP_061577075.1 competence type IV pilus ATPase ComGA Machinery gene
  NST70_RS09300 (NST70_09300) - 1959628..1960335 (+) 708 WP_014098020.1 helix-turn-helix domain-containing protein -
  NST70_RS09305 (NST70_09305) - 1960413..1960655 (+) 243 WP_014098019.1 DUF2626 domain-containing protein -
  NST70_RS09310 (NST70_09310) - 1960724..1961353 (-) 630 WP_035187941.1 MBL fold metallo-hydrolase -
  NST70_RS09315 (NST70_09315) - 1961483..1961656 (+) 174 WP_014098017.1 DUF2759 domain-containing protein -
  NST70_RS09320 (NST70_09320) - 1961961..1962929 (-) 969 WP_014098016.1 ROK family glucokinase -
  NST70_RS09325 (NST70_09325) - 1962922..1963143 (-) 222 WP_013859652.1 YqgQ family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 42374.92 Da        Isoelectric Point: 9.5494

>NTDB_id=982707 NST70_RS09295 WP_061577075.1 1958309..1959433(-) (comGA) [Weizmannia sp. FSL K6-0777]
MSVEKIAELLIGQAVKNNVTDVHIVPKEKHYHVQFRQYGRLYPHRNLSAKAGERLISHLKFMSSMDISEKRKPQSGSFAM
NVLQQTISLRISTLPTSLSKESLVIRILPHEEKFQLSQISLFPSSTKKIMALLNHSHGMLIFSGPTGSGKSTTMYTLVEH
CAKRFLRNVITLEDPVEKQSDSFLQVQVNEKAGITYSTGLKAILRHDPDIILVGEIRDAETARIAIRASLTGHLVLTTLH
TRDAKGAIYRLMEFGVSIHEMEQTLLAVSAQRLITLRCPVCGGIRCPHDSQDCPRSREKRQTAVYELLYGKNLQRVLKEA
KGEQVYYHYPTLKEWLRKGIVLGYISEEEYHRWIAEEEEADKPQTAGALSYPGR

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=982707 NST70_RS09295 WP_061577075.1 1958309..1959433(-) (comGA) [Weizmannia sp. FSL K6-0777]
ATGTCAGTTGAAAAAATTGCGGAACTTTTGATCGGACAGGCGGTAAAAAACAATGTGACCGATGTCCATATCGTTCCGAA
AGAAAAGCATTACCATGTCCAGTTCCGGCAGTACGGAAGGCTGTACCCCCACCGCAACCTTTCCGCGAAAGCCGGGGAAA
GGCTGATATCCCATTTGAAATTTATGTCTTCCATGGATATCAGTGAAAAACGGAAACCCCAAAGCGGGTCGTTCGCCATG
AACGTTTTGCAGCAGACGATCTCCTTAAGGATTTCGACGCTGCCAACCTCTCTTTCCAAAGAGAGCCTCGTTATTCGGAT
CTTGCCCCATGAAGAAAAATTCCAGCTCAGCCAAATTTCACTTTTTCCATCGAGCACAAAAAAAATAATGGCGCTGCTCA
ACCATTCGCATGGGATGCTGATTTTCAGCGGCCCGACTGGAAGCGGCAAGTCTACGACGATGTATACGCTTGTTGAGCAC
TGCGCAAAACGGTTTTTACGGAATGTGATTACACTGGAAGACCCTGTTGAAAAACAAAGCGACTCGTTTTTGCAGGTACA
GGTGAATGAAAAAGCAGGTATTACTTACAGCACAGGGCTGAAAGCGATTTTGCGCCATGATCCGGATATTATCCTGGTGG
GGGAAATCCGTGATGCCGAAACAGCAAGAATTGCGATCCGCGCATCACTCACCGGCCATCTCGTGCTGACGACATTGCAT
ACGCGTGACGCAAAGGGCGCGATTTACAGGCTGATGGAATTTGGGGTGAGCATCCACGAAATGGAGCAGACTTTGCTCGC
AGTCAGCGCACAGCGGCTGATTACATTGCGATGCCCGGTATGCGGTGGGATCCGCTGTCCGCATGATTCGCAAGATTGCC
CACGCAGCCGAGAAAAGCGGCAAACCGCAGTATATGAGCTGCTGTACGGGAAAAATTTGCAGCGAGTGTTGAAAGAAGCA
AAGGGGGAACAAGTCTACTATCATTATCCGACACTGAAAGAATGGCTCAGAAAGGGGATTGTGCTTGGATACATTTCTGA
AGAAGAGTATCACCGCTGGATTGCCGAAGAGGAAGAAGCGGATAAGCCTCAAACTGCAGGGGCGCTTTCTTACCCGGGTC
GGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

54.19

95.722

0.519

Multiple sequence alignment