Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NSQ51_RS00530 Genome accession   NZ_CP151966
Coordinates   102399..103769 (+) Length   456 a.a.
NCBI ID   WP_033017265.1    Uniprot ID   A0A150N5A2
Organism   Geobacillus sp. FSL K6-0789     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 97399..108769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ51_RS00510 (NSQ51_00510) ctsR 97771..98232 (+) 462 WP_033017268.1 transcriptional regulator CtsR -
  NSQ51_RS00515 (NSQ51_00515) - 98247..98795 (+) 549 WP_033008611.1 UvrB/UvrC motif-containing protein -
  NSQ51_RS00520 (NSQ51_00520) - 98800..99891 (+) 1092 WP_033017267.1 protein arginine kinase -
  NSQ51_RS00525 (NSQ51_00525) clpC 99888..102323 (+) 2436 WP_033017266.1 ATP-dependent protease ATP-binding subunit ClpC -
  NSQ51_RS00530 (NSQ51_00530) radA 102399..103769 (+) 1371 WP_033017265.1 DNA repair protein RadA Machinery gene
  NSQ51_RS00535 (NSQ51_00535) - 104055..105149 (+) 1095 WP_033017264.1 PIN/TRAM domain-containing protein -
  NSQ51_RS00540 (NSQ51_00540) ispD 105171..105857 (+) 687 WP_121649982.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NSQ51_RS00545 (NSQ51_00545) ispF 105872..106354 (+) 483 WP_033008623.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  NSQ51_RS00550 (NSQ51_00550) gltX 106461..107930 (+) 1470 WP_033008710.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49365.85 Da        Isoelectric Point: 7.7488

>NTDB_id=982479 NSQ51_RS00530 WP_033017265.1 102399..103769(+) (radA) [Geobacillus sp. FSL K6-0789]
MAKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIEPVRTAVRGAFLHSEPSGSAKPVPITAVAAVQEPRIETNSAEF
NRVLGGGIVKGSLVLIGGDPGIGKSTLLLQASAQLAARHTVLYVSGEESVKQVKLRAGRLRAESDHLYVLAEANLEYIVS
AIETIQPSCVVIDSIQTVYRTDITSAPGSVAQVRECTAELMKIAKTKGVAIFIVGHVTKEGAIAGPRILEHMVDTVLYFE
GERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLREVENPSEVFLEERSHGAAGSTVVAAMEGTRPVLVEIQALVSPTSFG
NPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDRPTNPTDVIIGEVGLTG
EVRRVSRIEQRVQEAVKLGFSRVIVPKNNLAGWQPPASVQVIGVSHVAEALEHTMI

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=982479 NSQ51_RS00530 WP_033017265.1 102399..103769(+) (radA) [Geobacillus sp. FSL K6-0789]
ATGGCGAAAAAGAAGACGAAATTTGTTTGCCAAGAGTGCGGATACGAATCGGCGAAGTGGCTCGGCCGCTGCCCGGGATG
CCAAACGTGGAACTCATTCGTCGAGGAAATCGAGCCGGTCAGAACCGCTGTGCGTGGGGCATTTCTCCATTCGGAGCCGT
CCGGGTCCGCTAAACCGGTTCCGATTACTGCCGTTGCCGCGGTGCAAGAGCCGCGCATCGAAACGAACAGTGCAGAGTTC
AATCGCGTGCTCGGTGGGGGGATCGTCAAAGGGTCGCTCGTCTTAATCGGCGGCGACCCCGGCATCGGCAAGTCGACGCT
GTTGCTGCAGGCCTCAGCGCAACTGGCGGCTAGGCATACAGTGTTGTATGTATCGGGCGAGGAATCCGTCAAACAGGTAA
AACTGCGCGCTGGGCGCCTTCGCGCCGAGTCCGATCATCTGTATGTGTTAGCGGAGGCGAATTTGGAATATATTGTATCA
GCGATTGAAACGATTCAGCCCTCCTGTGTCGTGATTGATTCAATCCAGACGGTGTACCGGACGGATATTACGTCAGCGCC
GGGCAGTGTCGCCCAAGTGCGCGAGTGCACCGCTGAGTTGATGAAAATCGCCAAAACAAAAGGCGTCGCCATTTTTATCG
TCGGCCATGTGACGAAAGAAGGGGCGATCGCCGGGCCGCGCATTCTCGAGCATATGGTCGATACCGTCCTTTATTTTGAG
GGGGAGCGGCATCATACGTACCGCATTTTGCGGGCGGTGAAAAACCGCTTCGGTTCGACGAATGAAATCGGTATTTTTGA
AATGCGCGACATCGGGCTGCGGGAAGTGGAAAATCCGTCCGAAGTGTTTTTGGAAGAGCGATCGCACGGGGCGGCGGGTT
CGACGGTGGTGGCGGCCATGGAAGGGACGCGCCCGGTTCTGGTGGAAATTCAAGCGCTCGTTTCGCCGACAAGCTTCGGC
AACCCGAGGCGGATGGCGACCGGCCTCGATCATAACCGCGTGTCGTTGCTTATGGCCGTATTGGAAAAGCGGGTCGGGCT
GCTGTTGCAAAACCAAGATGCCTATTTGAAGGTGGCGGGCGGAGTGAAGCTTGATGAGCCGGCCATTGACCTAGCCATCG
CCGTCAGCATCGCCTCAAGCTTTCGCGATCGGCCGACCAATCCGACCGACGTCATTATCGGTGAAGTCGGGTTGACCGGG
GAAGTGCGGCGTGTCTCCCGCATTGAACAGCGTGTGCAAGAGGCTGTCAAACTAGGTTTTTCCCGTGTCATTGTACCGAA
AAACAATTTGGCAGGTTGGCAGCCGCCGGCAAGCGTTCAGGTGATCGGCGTTTCCCATGTGGCTGAGGCGCTCGAGCACA
CGATGATTTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-221)

Subunit ChlI of Mg-chelatase (ChlI)

(364-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A150N5A2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.179

100

0.743
  radA Streptococcus mitis NCTC 12261

64.68

99.342

0.643
  radA Streptococcus mitis SK321

64.68

99.342

0.643
  radA Streptococcus pneumoniae Rx1

64.459

99.342

0.64
  radA Streptococcus pneumoniae D39

64.459

99.342

0.64
  radA Streptococcus pneumoniae R6

64.459

99.342

0.64
  radA Streptococcus pneumoniae TIGR4

64.459

99.342

0.64

Multiple sequence alignment