Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MHI30_RS13520 Genome accession   NZ_CP151961
Coordinates   2686679..2687449 (-) Length   256 a.a.
NCBI ID   WP_075752269.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0528     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2681679..2692449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI30_RS13500 (MHI30_13500) - 2681755..2682567 (-) 813 WP_020452237.1 pyruvate, water dikinase regulatory protein -
  MHI30_RS13505 (MHI30_13505) - 2682593..2683231 (-) 639 WP_025804968.1 helix-turn-helix transcriptional regulator -
  MHI30_RS13510 (MHI30_13510) glyS 2683428..2685467 (-) 2040 WP_075752272.1 glycine--tRNA ligase subunit beta -
  MHI30_RS13515 (MHI30_13515) glyQ 2685460..2686347 (-) 888 WP_075752270.1 glycine--tRNA ligase subunit alpha -
  MHI30_RS13520 (MHI30_13520) recO 2686679..2687449 (-) 771 WP_075752269.1 DNA repair protein RecO Machinery gene
  MHI30_RS13525 (MHI30_13525) - 2687487..2687630 (-) 144 WP_003183626.1 YqzL family protein -
  MHI30_RS13530 (MHI30_13530) era 2687792..2688697 (-) 906 WP_023855242.1 GTPase Era -
  MHI30_RS13535 (MHI30_13535) - 2688678..2689088 (-) 411 WP_065644190.1 cytidine deaminase -
  MHI30_RS13540 (MHI30_13540) - 2689221..2689601 (-) 381 WP_075752267.1 diacylglycerol kinase family protein -
  MHI30_RS13545 (MHI30_13545) ybeY 2689579..2690055 (-) 477 WP_075752265.1 rRNA maturation RNase YbeY -
  MHI30_RS13550 (MHI30_13550) - 2690052..2692106 (-) 2055 WP_237562614.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29497.14 Da        Isoelectric Point: 8.3124

>NTDB_id=982267 MHI30_RS13520 WP_075752269.1 2686679..2687449(-) (recO) [Bacillus sp. FSL L8-0528]
MLTKCEGIVLRTNDYGETNKIITLLTREHGKIGVLARGAKKPNSRLSAISQPFLYASFLFRKSSGLGTLEQGEMIESMRH
IREDLFLTAYAAYIAELIDKGTEEKKPNPFLFELLLQSMRRLDDGTDPDIIANLIEVKMLSVMGLYPELDECVHCKSKDG
TFHFSIRDNGFICHRCFAKDPYRIPLSPASARLLRLFFYFDLSRLGNVSVKQETKDELKRVIQTYYDEYSGIYLKSRRFM
EQMDNMKKMLGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=982267 MHI30_RS13520 WP_075752269.1 2686679..2687449(-) (recO) [Bacillus sp. FSL L8-0528]
ATGCTGACGAAATGTGAAGGTATCGTCCTTCGTACAAACGACTATGGTGAAACAAATAAAATCATTACGCTGTTGACGAG
AGAACACGGGAAAATCGGTGTGCTGGCAAGGGGCGCCAAAAAGCCGAACAGCCGCTTGTCAGCGATTTCCCAGCCGTTCC
TTTATGCTTCTTTTTTATTCCGCAAATCATCCGGTCTCGGCACATTGGAGCAGGGCGAGATGATCGAAAGCATGAGGCAT
ATACGCGAGGATCTGTTTTTAACAGCATATGCGGCCTACATCGCTGAATTAATCGACAAGGGAACGGAGGAAAAGAAACC
GAATCCGTTTTTATTTGAGCTGCTTCTTCAGTCGATGAGGAGGCTCGATGACGGTACAGATCCCGACATTATCGCCAACC
TTATCGAAGTTAAAATGCTCAGCGTGATGGGGCTGTATCCGGAGCTGGATGAATGTGTGCACTGCAAAAGCAAAGACGGC
ACATTTCATTTTTCGATCCGCGACAACGGCTTTATCTGCCACCGCTGTTTTGCGAAAGATCCATACAGGATACCGCTGTC
TCCCGCATCCGCCAGGCTGTTGAGGCTGTTTTTTTATTTTGACCTGTCAAGGCTCGGAAATGTCTCCGTCAAACAGGAGA
CGAAGGATGAGCTGAAAAGGGTCATTCAGACGTACTATGACGAGTACTCAGGGATCTACCTGAAATCGCGGCGCTTCATG
GAACAAATGGACAATATGAAAAAGATGCTGGGCGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

77.734

100

0.777

Multiple sequence alignment