Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MHI30_RS13150 Genome accession   NZ_CP151961
Coordinates   2622389..2623453 (-) Length   354 a.a.
NCBI ID   WP_075752345.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0528     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2617389..2628453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI30_RS13105 (MHI30_13105) tapA 2617883..2618611 (-) 729 WP_075752361.1 amyloid fiber anchoring/assembly protein TapA -
  MHI30_RS13110 (MHI30_13110) - 2618889..2619209 (+) 321 WP_075752359.1 YqzG/YhdC family protein -
  MHI30_RS13115 (MHI30_13115) - 2619239..2619421 (-) 183 WP_020452171.1 YqzE family protein -
  MHI30_RS13120 (MHI30_13120) comGG 2619510..2619875 (-) 366 WP_075752357.1 competence type IV pilus minor pilin ComGG -
  MHI30_RS13125 (MHI30_13125) comGF 2619887..2620375 (-) 489 WP_237562597.1 competence type IV pilus minor pilin ComGF -
  MHI30_RS13130 (MHI30_13130) comGE 2620284..2620631 (-) 348 WP_075752353.1 competence type IV pilus minor pilin ComGE -
  MHI30_RS13135 (MHI30_13135) comGD 2620615..2621052 (-) 438 WP_075752351.1 competence type IV pilus minor pilin ComGD -
  MHI30_RS13140 (MHI30_13140) comGC 2621058..2621351 (-) 294 WP_075752349.1 competence type IV pilus major pilin ComGC Machinery gene
  MHI30_RS13145 (MHI30_13145) comGB 2621365..2622399 (-) 1035 WP_075752347.1 competence type IV pilus assembly protein ComGB Machinery gene
  MHI30_RS13150 (MHI30_13150) comGA 2622389..2623453 (-) 1065 WP_075752345.1 competence type IV pilus ATPase ComGA Machinery gene
  MHI30_RS13155 (MHI30_13155) - 2623611..2624450 (-) 840 WP_075752343.1 STAS domain-containing protein -
  MHI30_RS13165 (MHI30_13165) - 2624692..2625069 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  MHI30_RS13170 (MHI30_13170) - 2625280..2625525 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  MHI30_RS13175 (MHI30_13175) - 2625561..2626199 (-) 639 WP_075752341.1 MBL fold metallo-hydrolase -
  MHI30_RS13180 (MHI30_13180) - 2626355..2626528 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  MHI30_RS13185 (MHI30_13185) - 2626589..2626903 (-) 315 WP_075752339.1 MTH1187 family thiamine-binding protein -
  MHI30_RS13190 (MHI30_13190) - 2626919..2628028 (-) 1110 WP_075752337.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39879.43 Da        Isoelectric Point: 8.9736

>NTDB_id=982266 MHI30_RS13150 WP_075752345.1 2622389..2623453(-) (comGA) [Bacillus sp. FSL L8-0528]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDRLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINRQPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLMLFTGPTGSGKTTTLYSLIEY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLEFGVDMTEIEQTLVAISAQRLVGLVCPFCGDSCSLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC
CDMKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=982266 MHI30_RS13150 WP_075752345.1 2622389..2623453(-) (comGA) [Bacillus sp. FSL L8-0528]
TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTCCGCTTCAGGATTGATGATGAACTTTTTCAAAAAGACAGATTAACGAGAAATGAGTGCT
CAAGGCTCATTTCTCACTTTAAATTCCTTTCTTCGATGGACATTGGGGAAAGACGGCTGCCGCAAAGCGGAGCTTTAACT
CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTTCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATGCTGTTCACCGGTCCGACAGGCTCGGGGAAAACGACAACACTCTATTCTTTAATTGAATAC
GCTAAGCGGCATTTTAAGCGAAATATTATTACCTTGGAAGACCCTGTCGAATCAAGAAGCGAAAACATTTTGCAAGTGCA
AGTGAATGAAAAAGCGGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTTCGCCACGATCCGGACATGATCATCCTTG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACAGGACACCTTGTTTTATCGAGCATGCAT
GCGAAAAATGCAAAAGGCGCGATATACAGGCTGCTCGAGTTCGGAGTTGATATGACAGAAATTGAACAAACACTGGTCGC
AATCAGCGCCCAACGCCTCGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAGCTGCTCTTTATATTGCAGATTGTCGAGGC
CTGTGAGAAGAGCAAGCGTCTTCGAACTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAAGAAGCAAAAGGCAGGTGC
TGTGACATGAAAACAGAAACTTTGAAAACGCTGATCCAGAAGGGAATAGCACTCGGATATCTGCCGTCGAATACTTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.797

100

0.678

Multiple sequence alignment