Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MHI30_RS00600 Genome accession   NZ_CP151961
Coordinates   111156..112535 (+) Length   459 a.a.
NCBI ID   WP_020449839.1    Uniprot ID   A0AAW6KF37
Organism   Bacillus sp. FSL L8-0528     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 106156..117535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI30_RS00580 (MHI30_00580) - 106523..106987 (+) 465 WP_075752574.1 CtsR family transcriptional regulator -
  MHI30_RS00585 (MHI30_00585) - 107002..107556 (+) 555 WP_075752572.1 UvrB/UvrC motif-containing protein -
  MHI30_RS00590 (MHI30_00590) - 107556..108647 (+) 1092 WP_075752570.1 protein arginine kinase -
  MHI30_RS00595 (MHI30_00595) clpC 108644..111076 (+) 2433 WP_075752568.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MHI30_RS00600 (MHI30_00600) radA 111156..112535 (+) 1380 WP_020449839.1 DNA repair protein RadA Machinery gene
  MHI30_RS00605 (MHI30_00605) disA 112539..113615 (+) 1077 WP_020449840.1 DNA integrity scanning diadenylate cyclase DisA -
  MHI30_RS00610 (MHI30_00610) - 113747..114835 (+) 1089 WP_009330350.1 PIN/TRAM domain-containing protein -
  MHI30_RS00615 (MHI30_00615) ispD 114852..115547 (+) 696 WP_075752564.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MHI30_RS00620 (MHI30_00620) ispF 115540..116016 (+) 477 WP_075752562.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49848.28 Da        Isoelectric Point: 7.4339

>NTDB_id=982227 MHI30_RS00600 WP_020449839.1 111156..112535(+) (radA) [Bacillus sp. FSL L8-0528]
MAKTKTKFICHSCGYESAKWMGKCPGCGTWNTMVEETIKKPAANRRTAFSHSVQTVQKPSPLTSIETTEEPRIKTKLGEF
NRVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADSKENVLYISGEESVKQTKLRADRLGINSPTLHVLSETDMEYIT
SSIEEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPHPTDCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMIIPSANVEGWTKPKGIEVVGVENVAEALRASLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=982227 MHI30_RS00600 WP_020449839.1 111156..112535(+) (radA) [Bacillus sp. FSL L8-0528]
ATGGCTAAAACAAAGACTAAATTCATTTGTCACTCATGCGGTTACGAATCCGCCAAGTGGATGGGGAAGTGCCCCGGATG
CGGCACATGGAATACCATGGTGGAAGAAACAATAAAAAAACCCGCCGCCAACAGAAGAACCGCTTTTTCACATTCCGTTC
AAACGGTGCAAAAGCCTTCACCTCTCACTTCAATCGAAACAACAGAAGAGCCGCGAATTAAAACGAAACTGGGCGAATTC
AACCGCGTCTTGGGAAACGGTATTGTCAAAGGTTCACTCGTTTTAATCGGAGGCGATCCCGGCATCGGGAAATCAACCTT
GCTGCTTCAAGTATCTGCCCAGCTCGCTGATTCAAAAGAAAATGTCCTGTACATCTCAGGTGAAGAATCGGTCAAGCAGA
CAAAGCTGAGAGCAGACCGTCTAGGCATCAACAGTCCCACTCTTCACGTTTTATCTGAAACCGATATGGAGTATATTACG
TCTTCTATAGAAGAGATGAATCCATCATTCGTGGTGGTTGATTCGATACAAACCGTTTACCAAAGTGATATTACATCCGC
TCCGGGCAGCGTGTCCCAGGTCAGGGAATGCACCGCTGAGCTGATGAGAATTGCAAAAACAAAAGGGATTCCGATATTTA
TCGTCGGGCATGTCACAAAAGAAGGTTCGATTGCCGGACCGAGACTTCTGGAACATATGGTCGACACCGTCCTTTACTTT
GAAGGTGAGCGGCATCATACATTTCGGATTTTAAGAGCCGTCAAAAACCGGTTTGGATCAACGAATGAAATGGGAATCTT
TGAAATGAGGGAAGAGGGCCTGACAGAAGTGCTGAATCCGTCGGAAATCTTTCTCGAAGAGCGCTCGGCGGGAGCAGCCG
GCTCGAGTGTCGTGGCTTCAATGGAAGGCACGAGGCCGGTCTTAGTAGAGATTCAGGCGCTGATTTCCCCCACGAGTTTT
GGAAATCCGCGCAGGATGGCAACCGGAATTGATCATAACCGCGTCTCATTGCTGATGGCGGTTTTAGAAAAAAGGGTTGG
GCTGCTGCTGCAAAATCAAGACGCCTATTTAAAAGTCGCCGGCGGCGTCAAGCTGGACGAGCCGGCGATCGACCTCGCCG
TTGCGGTGAGCATCGCCTCAAGCTTCAGAGACACCCCGCCTCATCCGACGGATTGTTTTATCGGAGAAGTCGGCTTGACA
GGGGAAGTCCGCAGAGTATCAAGGATAGAACAGAGGGTGCAGGAAGCGGCGAAGCTTGGTTTTAAAAGAATGATTATTCC
TTCTGCAAATGTGGAAGGATGGACAAAGCCGAAAGGAATTGAAGTCGTCGGCGTTGAAAATGTAGCTGAGGCCCTTCGAG
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

91.048

99.782

0.908
  radA Streptococcus mitis NCTC 12261

62.031

98.693

0.612
  radA Streptococcus pneumoniae Rx1

62.031

98.693

0.612
  radA Streptococcus pneumoniae D39

62.031

98.693

0.612
  radA Streptococcus pneumoniae R6

62.031

98.693

0.612
  radA Streptococcus pneumoniae TIGR4

62.031

98.693

0.612
  radA Streptococcus mitis SK321

61.81

98.693

0.61

Multiple sequence alignment