Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   MHI30_RS00020 Genome accession   NZ_CP151961
Coordinates   3055..4167 (+) Length   370 a.a.
NCBI ID   WP_075751816.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0528     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI30_RS00005 (MHI30_00005) dnaA 1..1341 (+) 1341 WP_075751815.1 chromosomal replication initiator protein DnaA -
  MHI30_RS00010 (MHI30_00010) dnaN 1519..2655 (+) 1137 WP_026580417.1 DNA polymerase III subunit beta -
  MHI30_RS00015 (MHI30_00015) yaaA 2823..3038 (+) 216 WP_003178084.1 S4 domain-containing protein YaaA -
  MHI30_RS00020 (MHI30_00020) recF 3055..4167 (+) 1113 WP_075751816.1 DNA replication/repair protein RecF Machinery gene
  MHI30_RS00025 (MHI30_00025) - 4185..4427 (+) 243 WP_020449765.1 extracellular matrix/biofilm biosynthesis regulator RemA family protein -
  MHI30_RS00030 (MHI30_00030) gyrB 4488..6401 (+) 1914 WP_075751817.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  MHI30_RS00035 (MHI30_00035) gyrA 6592..9066 (+) 2475 WP_075751818.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42422.29 Da        Isoelectric Point: 6.7305

>NTDB_id=982223 MHI30_RS00020 WP_075751816.1 3055..4167(+) (recF) [Bacillus sp. FSL L8-0528]
MYIQNLTLSSYRNYERLDLQFENKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDKELIRWDEDYAKIEGRVIKKNGS
VPIQLVISKKGKKGKVNHIEQQKLSQYVGAVNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDQTMLDVLTEQLTEFAAKVVMKRLQFVDQLEKWAQPIHSGISRGLEELTLKYHTSLHVSDSPDLSKM
INSYQETFSKLRDKEIERGVSLSGPHRDDVLFCVNGRDVQTYGSQGQQRTTALSLKLAEIDLIQEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHKTLNEAEIFRVENGTLSD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=982223 MHI30_RS00020 WP_075751816.1 3055..4167(+) (recF) [Bacillus sp. FSL L8-0528]
TTGTATATCCAAAATCTAACATTATCGTCTTACCGTAATTATGAGCGCCTTGACCTTCAATTTGAGAACAAAGTGAACGT
GATTATCGGAGAGAACGCTCAAGGAAAAACGAATTTGATGGAAGCGATCTATGTGCTTGCGATGGCCAAATCCCATCGGA
CGTCAAATGATAAAGAACTCATACGATGGGATGAAGACTATGCTAAAATAGAAGGCAGGGTCATTAAAAAAAACGGTTCT
GTTCCCATTCAGCTCGTGATTTCCAAAAAAGGAAAAAAAGGTAAAGTCAATCATATTGAACAGCAGAAGCTGAGCCAGTA
TGTCGGGGCTGTCAACACGATTATGTTCGCGCCGGAGGATTTAAATCTTGTAAAAGGAAGCCCTCAGGTCAGAAGAAGGT
TTCTCGATATGGAAATCGGCCAAGTATCACCTGTCTATCTTCATGACCTTTCTCTTTACCAAAAAATCCTTTCACAACGG
AATCATTTCCTGAAACAGCTTCAAACGAGAAAGCAAACCGATCAAACGATGCTTGACGTGCTGACGGAACAGCTTACGGA
ATTCGCGGCAAAGGTTGTGATGAAACGGCTTCAGTTTGTCGATCAGCTTGAAAAATGGGCTCAGCCCATTCATTCCGGAA
TTTCAAGAGGTCTGGAAGAGCTGACGTTAAAGTATCATACGTCTCTTCACGTATCAGATTCGCCCGATTTGTCGAAAATG
ATCAATAGTTATCAAGAAACGTTTTCTAAATTAAGAGATAAAGAAATAGAACGGGGGGTATCTCTGTCAGGTCCCCACAG
GGATGATGTTCTCTTCTGCGTCAATGGCCGTGATGTGCAGACTTACGGATCGCAGGGCCAGCAGCGAACGACTGCATTGT
CGCTTAAATTGGCTGAAATCGATTTGATTCAGGAAGAGATAGGAGAATATCCGATTCTGCTTTTGGATGATGTTTTATCA
GAGCTGGACGACTATCGGCAGTCTCACTTGCTCCACACCATTCAAGGCCGTGTTCAAACATTCGTAACAACGACAAGCGT
TGATGGAATTGATCATAAAACCTTAAACGAAGCGGAAATCTTTCGAGTTGAAAATGGCACGCTATCGGACTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

88.859

99.459

0.884

Multiple sequence alignment