Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NST20_RS08915 Genome accession   NZ_CP151954
Coordinates   1813221..1814345 (-) Length   374 a.a.
NCBI ID   WP_013859647.1    Uniprot ID   -
Organism   Weizmannia sp. FSL W8-0676     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1808221..1819345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST20_RS08870 (NST20_08870) - 1808791..1809582 (+) 792 WP_342399300.1 YqhG family protein -
  NST20_RS08875 (NST20_08875) - 1809616..1809792 (-) 177 WP_064485109.1 YqzE family protein -
  NST20_RS08880 (NST20_08880) - 1809779..1810336 (-) 558 WP_342399301.1 shikimate kinase -
  NST20_RS08885 (NST20_08885) comGG 1810333..1810701 (-) 369 WP_029142070.1 competence type IV pilus minor pilin ComGG -
  NST20_RS08890 (NST20_08890) comGF 1810698..1811180 (-) 483 WP_342399302.1 competence type IV pilus minor pilin ComGF -
  NST20_RS08895 (NST20_08895) - 1811143..1811451 (-) 309 WP_029142068.1 type II secretion system protein -
  NST20_RS08900 (NST20_08900) comGD 1811438..1811881 (-) 444 WP_029142067.1 competence type IV pilus minor pilin ComGD -
  NST20_RS08905 (NST20_08905) comGC 1811878..1812189 (-) 312 WP_013859645.1 competence type IV pilus major pilin ComGC -
  NST20_RS08910 (NST20_08910) comGB 1812257..1813321 (-) 1065 WP_342399303.1 competence type IV pilus assembly protein ComGB -
  NST20_RS08915 (NST20_08915) comGA 1813221..1814345 (-) 1125 WP_013859647.1 competence type IV pilus ATPase ComGA Machinery gene
  NST20_RS08920 (NST20_08920) - 1814540..1815247 (+) 708 WP_258921440.1 helix-turn-helix domain-containing protein -
  NST20_RS08925 (NST20_08925) - 1815325..1815567 (+) 243 WP_014098019.1 DUF2626 domain-containing protein -
  NST20_RS08930 (NST20_08930) - 1815637..1816266 (-) 630 WP_013859649.1 MBL fold metallo-hydrolase -
  NST20_RS08935 (NST20_08935) - 1816396..1816569 (+) 174 WP_019720590.1 DUF2759 domain-containing protein -
  NST20_RS08940 (NST20_08940) - 1816668..1817636 (-) 969 WP_342399304.1 ROK family glucokinase -
  NST20_RS08945 (NST20_08945) - 1817629..1817850 (-) 222 WP_342399305.1 YqgQ family protein -
  NST20_RS08950 (NST20_08950) - 1818159..1819325 (-) 1167 WP_041819143.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 42378.89 Da        Isoelectric Point: 9.4407

>NTDB_id=981999 NST20_RS08915 WP_013859647.1 1813221..1814345(-) (comGA) [Weizmannia sp. FSL W8-0676]
MSVEKIAELLIGQAVKNNVTDVHIVPKEKHYHVQFRQYGRLYPHRNLSAKAGERLISHLKFMSSMDISEKRKPQSGSFAM
NVLQQTISLRISTLPTSLSKESLVIRILPHEEQFQLSQISLFPSSTKKLMALLNHSHGMLIFSGPTGSGKSTTMYTLVEH
CAKRFLRNVITLEDPVEKQSDSFLQVQVNEKAGITYSTGLKAILRHDPDIILVGEIRDAETARIAVRASLTGHLVLTTLH
TRDAKGAIYRMMEFGVSIHEMEQTLLAVSAQRLITLRCPVCGGIRCPHDSQDCPRSREKRQTAVYELLYGKNLQRVLKEA
KGEQVYYHYPTLKEWLRKGIVLGYISEEEYHRWIAEEEEADKPQTAGALSYPGR

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=981999 NST20_RS08915 WP_013859647.1 1813221..1814345(-) (comGA) [Weizmannia sp. FSL W8-0676]
ATGTCAGTTGAAAAAATTGCGGAACTTTTGATCGGACAGGCGGTAAAAAACAATGTGACCGATGTCCATATCGTTCCGAA
AGAAAAGCATTACCATGTCCAGTTCCGGCAGTACGGAAGGCTGTACCCCCACCGCAACCTTTCCGCGAAAGCCGGGGAAA
GGCTGATATCCCATTTGAAATTTATGTCTTCCATGGATATCAGTGAAAAACGGAAACCCCAAAGCGGGTCGTTTGCCATG
AACGTTTTGCAGCAGACGATCTCCTTAAGGATTTCGACGCTGCCAACCTCTCTTTCCAAAGAGAGCCTCGTCATCCGGAT
TTTGCCCCACGAAGAACAATTCCAGCTCAGCCAAATTTCACTTTTTCCATCGAGCACGAAAAAATTAATGGCGCTATTAA
ACCATTCGCACGGCATGCTGATTTTCAGCGGTCCGACCGGAAGCGGCAAGTCGACGACGATGTATACGCTTGTTGAGCAC
TGCGCAAAACGGTTTTTACGGAATGTAATTACACTGGAAGACCCTGTTGAAAAACAAAGCGATTCGTTTTTGCAGGTGCA
GGTGAATGAAAAAGCAGGCATTACTTATAGCACAGGGCTGAAAGCAATTTTGCGCCATGATCCGGATATTATCCTGGTGG
GGGAAATCCGCGATGCCGAAACAGCAAGAATTGCGGTCCGCGCATCGCTCACCGGCCATCTCGTGCTGACGACATTGCAT
ACGCGTGACGCAAAGGGCGCGATCTACAGGATGATGGAATTCGGCGTGAGCATCCACGAAATGGAGCAGACTTTGCTCGC
AGTCAGCGCACAGCGGCTGATTACATTGCGATGCCCGGTATGCGGTGGGATCCGCTGTCCGCATGATTCGCAAGATTGCC
CACGCAGCCGAGAAAAGCGGCAAACCGCGGTATATGAGCTGCTGTACGGGAAAAATTTGCAGCGAGTGTTGAAAGAAGCA
AAGGGGGAACAAGTCTACTATCATTATCCGACACTGAAAGAATGGCTCAGAAAGGGGATTGTGCTTGGATACATTTCTGA
AGAAGAGTATCACCGCTGGATTGCCGAAGAGGAAGAAGCGGATAAGCCTCAAACTGCAGGGGCGCTTTCTTACCCGGGTC
GGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

54.469

95.722

0.521

Multiple sequence alignment