Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACKUF0_RS27365 Genome accession   NZ_CP183105
Coordinates   5138355..5139788 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain 463     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5133355..5144788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUF0_RS27335 (ACKUF0_27295) yrbG 5133912..5134890 (+) 979 Protein_4978 calcium/sodium antiporter -
  ACKUF0_RS27340 (ACKUF0_27300) kdsD 5134904..5135890 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACKUF0_RS27345 (ACKUF0_27305) kdsC 5135911..5136477 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACKUF0_RS27350 (ACKUF0_27310) lptC 5136474..5137049 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACKUF0_RS27355 (ACKUF0_27315) lptA 5137018..5137575 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACKUF0_RS27360 (ACKUF0_27320) lptB 5137582..5138307 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACKUF0_RS27365 (ACKUF0_27325) rpoN/rpoN1 5138355..5139788 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACKUF0_RS27370 (ACKUF0_27330) hpf 5139811..5140098 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACKUF0_RS27375 (ACKUF0_27335) ptsN 5140216..5140707 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACKUF0_RS27380 (ACKUF0_27340) rapZ 5140753..5141607 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ACKUF0_RS27385 (ACKUF0_27345) npr 5141604..5141876 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACKUF0_RS27390 (ACKUF0_27350) yrbL 5142089..5142721 (+) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACKUF0_RS27395 (ACKUF0_27355) mtgA 5142718..5143446 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACKUF0_RS27400 (ACKUF0_27360) elbB 5143443..5144096 (-) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=981978 ACKUF0_RS27365 WP_000809051.1 5138355..5139788(+) (rpoN/rpoN1) [Escherichia coli strain 463]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=981978 ACKUF0_RS27365 WP_000809051.1 5138355..5139788(+) (rpoN/rpoN1) [Escherichia coli strain 463]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAAGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGATGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCGATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
AGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGCCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAATGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAA
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465