Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   ACKUFX_RS08325 Genome accession   NZ_CP183089
Coordinates   1620896..1622278 (-) Length   460 a.a.
NCBI ID   WP_001295751.1    Uniprot ID   -
Organism   Escherichia coli strain 447     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1615896..1627278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKUFX_RS08315 (ACKUFX_08295) ettA 1617855..1619522 (+) 1668 WP_000046754.1 energy-dependent translational throttle protein EttA -
  ACKUFX_RS08320 (ACKUFX_08300) nadR 1619643..1620875 (-) 1233 WP_000093834.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  ACKUFX_RS08325 (ACKUFX_08305) radA/sms 1620896..1622278 (-) 1383 WP_001295751.1 DNA repair protein RadA Machinery gene
  ACKUFX_RS08330 (ACKUFX_08310) serB 1622327..1623295 (-) 969 WP_001132966.1 phosphoserine phosphatase -
  ACKUFX_RS08335 (ACKUFX_08315) ytjB 1623401..1624045 (+) 645 WP_000124608.1 YtjB family periplasmic protein -
  ACKUFX_RS08340 (ACKUFX_08320) lplA 1624073..1625089 (+) 1017 WP_000105853.1 lipoate--protein ligase LplA -
  ACKUFX_RS08345 (ACKUFX_08325) yjjJ 1625090..1626421 (-) 1332 WP_001293116.1 type II toxin-antitoxin system HipA family toxin YjjJ -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49472.04 Da        Isoelectric Point: 7.1936

>NTDB_id=981745 ACKUFX_RS08325 WP_001295751.1 1620896..1622278(-) (radA/sms) [Escherichia coli strain 447]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKTPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=981745 ACKUFX_RS08325 WP_001295751.1 1620896..1622278(-) (radA/sms) [Escherichia coli strain 447]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCAGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGAGTGGCAAAAGTCCAGAAACTTTCCGACATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGCGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCGCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTTAATATGCTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGCAGCGTGGCACAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGTGTCTTTGCAATGACCGAGCAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTAGTGTGGGAAGGAACGCGTCCGCTGCTGGTGGAGATTCAGGCGCTCGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTGGCAGTCGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTTCACCG
TCACGGTGGTCTACAAATGGCCGATCAGGATGTGTTTGTTAACGTGGTCGGTGGCGTGAAGGTGACGGAAACCAGTGCCG
ATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTACCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAACGAATCTCAGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAACGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

47.046

99.348

0.467
  radA Streptococcus mitis SK321

44.079

99.13

0.437
  radA Streptococcus pneumoniae D39

43.64

99.13

0.433
  radA Streptococcus pneumoniae TIGR4

43.64

99.13

0.433
  radA Streptococcus mitis NCTC 12261

43.64

99.13

0.433
  radA Streptococcus pneumoniae R6

43.64

99.13

0.433
  radA Streptococcus pneumoniae Rx1

43.64

99.13

0.433