Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   R8625_RS00145 Genome accession   NZ_AP026923
Coordinates   25078..26439 (+) Length   453 a.a.
NCBI ID   WP_074017595.1    Uniprot ID   A0A237IXT5
Organism   Streptococcus pneumoniae strain PZ900700054     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20078..31439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8625_RS00105 (PC0054_00150) - 20224..20620 (+) 397 Protein_14 IS630 family transposase -
  R8625_RS00110 - 20756..21230 (-) 475 Protein_15 hypothetical protein -
  R8625_RS00115 (PC0054_00170) comW 21496..21732 (+) 237 WP_000939546.1 sigma(X)-activator ComW Regulator
  R8625_RS00120 (PC0054_00180) - 21963..23249 (+) 1287 WP_000205044.1 adenylosuccinate synthase -
  R8625_RS00125 (PC0054_00190) tadA 23450..23917 (+) 468 WP_000291870.1 tRNA adenosine(34) deaminase TadA -
  R8625_RS00135 (PC0054_00200) - 24104..24547 (+) 444 WP_000701992.1 dUTP diphosphatase -
  R8625_RS00140 (PC0054_00210) - 24549..25064 (+) 516 WP_001823418.1 histidine phosphatase family protein -
  R8625_RS00145 (PC0054_00220) radA 25078..26439 (+) 1362 WP_074017595.1 DNA repair protein RadA Machinery gene
  R8625_RS00150 (PC0054_00230) - 26512..27009 (+) 498 WP_317649200.1 carbonic anhydrase -
  R8625_RS00155 (PC0054_00240) - 27034..27849 (+) 816 WP_317649201.1 PrsW family intramembrane metalloprotease -
  R8625_RS00160 (PC0054_00250) - 27994..28962 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  R8625_RS00165 - 29080..30025 (+) 946 Protein_25 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.95 Da        Isoelectric Point: 6.5137

>NTDB_id=98163 R8625_RS00145 WP_074017595.1 25078..26439(+) (radA) [Streptococcus pneumoniae strain PZ900700054]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=98163 R8625_RS00145 WP_074017595.1 25078..26439(+) (radA) [Streptococcus pneumoniae strain PZ900700054]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTTAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGATTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTCTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8RXC

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

100

100

1
  radA Streptococcus pneumoniae D39

100

100

1
  radA Streptococcus pneumoniae R6

100

100

1
  radA Streptococcus pneumoniae TIGR4

100

100

1
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment