Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACNM7T_RS16705 Genome accession   NZ_CP183041
Coordinates   3372855..3374165 (-) Length   436 a.a.
NCBI ID   WP_416806565.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Scm     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3367855..3379165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNM7T_RS16685 (ACNM7T_16685) - 3368095..3368811 (-) 717 WP_100001619.1 (Fe-S)-binding protein -
  ACNM7T_RS16690 (ACNM7T_16690) - 3368995..3370185 (-) 1191 WP_085342334.1 PLP-dependent aminotransferase family protein -
  ACNM7T_RS16695 (ACNM7T_16695) - 3370216..3370935 (-) 720 WP_015240627.1 FadR/GntR family transcriptional regulator -
  ACNM7T_RS16700 (ACNM7T_16700) lutP 3371141..3372817 (+) 1677 WP_015240628.1 L-lactate permease LutP -
  ACNM7T_RS16705 (ACNM7T_16705) rpoN/rpoN1 3372855..3374165 (-) 1311 WP_416806565.1 RNA polymerase factor sigma-54 Machinery gene
  ACNM7T_RS16710 (ACNM7T_16710) yvfG 3374243..3374461 (+) 219 WP_003151570.1 protein YvfG -
  ACNM7T_RS16715 (ACNM7T_16715) - 3374475..3375440 (-) 966 WP_216061894.1 polysaccharide pyruvyl transferase family protein -
  ACNM7T_RS16720 (ACNM7T_16720) - 3375419..3376591 (-) 1173 WP_017418873.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  ACNM7T_RS16725 (ACNM7T_16725) - 3376596..3377243 (-) 648 WP_094031310.1 acetyltransferase -
  ACNM7T_RS16730 (ACNM7T_16730) - 3377240..3377848 (-) 609 WP_094031311.1 sugar transferase -

Sequence

Protein


Download         Length: 436 a.a.        Molecular weight: 49860.90 Da        Isoelectric Point: 9.3800

>NTDB_id=981622 ACNM7T_RS16705 WP_416806565.1 3372855..3374165(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain Scm]
MDIKLQQTQMLKPQLTQELKQAITLLGYNSAELAGYIDELSLENPLIERKETDTPPLSYHKTSKNRMKERDGSQQIRLTR
KTLQDSLKQQALDMKLSDDEKKIFNYLIHSLDSNGYLKEDIPAAADHVSASQEETEAVLKKLQSLEPAGIGARSLQECIL
LQLRRLPERNEQAEWIADAYFELFARKKWKALTLKTGIPLKDIQDIADMISALDPRPGLRYAHEEPGFYVEPDIFITVKN
GIIQARLNSRSFPDIQLHTHYQPLLSAAGCRDTKNYLSAKYQEWRWLNRALSQRKQTITRIIGVLTERQESFFLTGKSGM
QPLTLREVAETLELHESTISRAIKGKYVQTPYGLYEMKTFFSAKAESSGDGGASNYAVKAHLEALVGKEDKKKPFSDQKL
ADLLHEQYDIRISRRTVAKYRDQLNIPPSSARKRYS

Nucleotide


Download         Length: 1311 bp        

>NTDB_id=981622 ACNM7T_RS16705 WP_416806565.1 3372855..3374165(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain Scm]
ATGGATATCAAACTTCAGCAAACACAAATGTTAAAGCCTCAATTAACGCAAGAGCTTAAACAGGCTATCACATTACTCGG
ATACAATTCCGCGGAACTGGCCGGATATATTGATGAGCTCTCACTGGAAAACCCTCTGATTGAACGAAAGGAAACAGACA
CGCCGCCACTATCTTACCATAAAACAAGTAAAAACAGGATGAAAGAACGGGACGGAAGCCAGCAGATCAGACTTACCCGC
AAAACGCTGCAGGATTCGCTCAAACAGCAAGCCTTAGATATGAAGCTGTCCGATGATGAGAAAAAGATTTTCAATTATCT
GATACATTCATTGGACTCAAACGGATATTTAAAAGAGGACATACCAGCTGCCGCCGACCATGTATCCGCTTCACAAGAGG
AGACGGAAGCGGTTCTGAAAAAGCTTCAATCCCTTGAGCCCGCGGGAATCGGCGCCCGGTCTTTGCAGGAATGCATCCTT
CTTCAGCTCCGCCGTCTTCCCGAGCGGAACGAACAGGCGGAATGGATTGCCGACGCCTATTTTGAGCTATTTGCAAGAAA
GAAATGGAAAGCGCTTACACTGAAAACGGGCATTCCGCTTAAAGACATTCAGGACATCGCTGACATGATCAGCGCCCTTG
ATCCGAGGCCGGGGCTCCGTTATGCGCATGAGGAGCCGGGTTTTTACGTGGAACCGGATATTTTCATCACAGTGAAAAAC
GGCATCATTCAAGCGCGGCTCAACAGCCGTTCGTTTCCTGACATCCAGCTTCATACCCATTATCAGCCGCTGCTGTCAGC
TGCCGGGTGCCGTGACACGAAAAATTATCTGTCGGCAAAATATCAGGAATGGCGCTGGCTGAACCGGGCTTTGTCGCAAA
GAAAACAAACGATCACGAGAATTATCGGCGTCCTGACTGAACGGCAGGAATCCTTTTTTCTCACAGGAAAAAGCGGAATG
CAGCCGCTGACCTTAAGAGAGGTGGCGGAAACGCTGGAGCTCCACGAATCAACGATCAGCCGGGCCATCAAAGGGAAGTA
CGTTCAGACGCCGTACGGACTGTATGAAATGAAAACATTTTTCTCGGCGAAAGCTGAATCGTCGGGGGACGGCGGCGCCT
CCAATTATGCCGTAAAAGCGCATCTGGAGGCATTGGTCGGTAAAGAAGATAAAAAGAAACCTTTTTCAGACCAAAAGCTT
GCCGATCTGCTGCATGAACAATATGACATCCGAATCTCAAGGCGAACCGTCGCCAAATACCGTGACCAATTAAATATTCC
GCCGTCATCGGCGAGAAAACGGTACAGCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(77-261)

Sigma-54, DNA binding domain (Sigma54_DBD)

(275-434)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

33.267

100

0.383

Multiple sequence alignment