Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   NSS81_RS04945 Genome accession   NZ_CP151943
Coordinates   929777..932131 (-) Length   784 a.a.
NCBI ID   WP_342432428.1    Uniprot ID   -
Organism   Neobacillus sp. FSL H8-0543     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 924777..937131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSS81_RS04930 (NSS81_04930) dtd 926366..926809 (-) 444 WP_342432425.1 D-aminoacyl-tRNA deacylase -
  NSS81_RS04935 (NSS81_04935) - 926825..929020 (-) 2196 WP_342432426.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  NSS81_RS04940 (NSS81_04940) - 929265..929777 (-) 513 WP_342432427.1 adenine phosphoribosyltransferase -
  NSS81_RS04945 (NSS81_04945) recJ 929777..932131 (-) 2355 WP_342432428.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  NSS81_RS04950 (NSS81_04950) secDF 932243..934507 (-) 2265 WP_342432429.1 protein translocase subunit SecDF -
  NSS81_RS04955 (NSS81_04955) - 934666..934962 (-) 297 WP_342432430.1 post-transcriptional regulator -
  NSS81_RS04960 (NSS81_04960) spoVB 935098..936654 (+) 1557 WP_342432431.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 784 a.a.        Molecular weight: 87866.06 Da        Isoelectric Point: 5.3257

>NTDB_id=981519 NSS81_RS04945 WP_342432428.1 929777..932131(-) (recJ) [Neobacillus sp. FSL H8-0543]
MLKSKTRWIVRESDSQLVKTLEKELNITPLVASMLINRGIDTVESAQFFLYGKEQFHDPYLLKGMDLAVIRIREAIDNQE
PILIYGDYDADGVSSTSVLMITLMELGANVQFYIPNRFTEGYGPNEPAFRHAAETGIKLIITVDTGISAIHEAKVAKELG
VDLIITDHHEPGPELPEALAIIHPKLPDSIYPFRELAGVGVAFKLAHALYEVIPEHLFEIAVIGTVADLVSLKDENRLLV
KKGLEKLKVTQNIGLKAILKLAGVNSQAITEETIGFTLAPRINAVGRLENADLAVQLLLTNDPNEAQSLAAEMDALNKQR
QSIVNSITIEAIEEVENNFPIDSNSVLVIGKEGWNAGVIGIVASRLVEKYYRPTIVLSFDKEKGLAKGSARSIAGFDLFE
NLSTCRDILPHFGGHPMAAGMTLNLEDVSLLRSRLNKLANEQLTDEDFIAVTSLDHEITLDEINLPTLEELNLLAPFGMD
NPKPKVLISDVQISSIRKIGSDQNHLKVLVNDSGVNLDGIGFGLGTLVDQISPAAKISIIGELSVNEWNNIRKPQIFIQD
IAVDTWQLFDHRGVKRIRLMDGTIPKEKRKFIIFNKEQLDKADPALKDEITYIHDVEVAKNFNSKNANIVLMDFPPSKEI
LIHLFKGKKPARIYAYFQKENSDFFSTIPTRDHFKWFYALLMKKGPIDLGRYGEVIAKKRGWSQETITFMSRVFSELEFV
TMNKGFITLNKQAHKRDLTDSHTYQTKQAQFALERDLLFSSFQDLKNWFDDVIEEPVITEEAIK

Nucleotide


Download         Length: 2355 bp        

>NTDB_id=981519 NSS81_RS04945 WP_342432428.1 929777..932131(-) (recJ) [Neobacillus sp. FSL H8-0543]
ATGTTAAAGTCGAAAACGAGATGGATTGTTCGTGAATCTGATAGTCAACTAGTCAAAACACTTGAAAAAGAATTGAATAT
AACACCACTTGTTGCGTCGATGCTTATTAACCGCGGAATAGATACTGTTGAGTCTGCACAGTTTTTTTTATATGGTAAGG
AACAATTTCATGATCCCTATTTACTAAAAGGTATGGATTTAGCTGTTATAAGAATCCGTGAGGCAATTGATAATCAAGAA
CCAATCCTCATATATGGTGACTATGACGCCGATGGTGTTAGCAGCACCTCGGTATTAATGATTACCCTCATGGAATTAGG
AGCAAACGTTCAATTCTATATACCTAACCGTTTTACGGAAGGCTATGGCCCTAATGAGCCAGCATTTCGTCATGCAGCTG
AAACAGGGATAAAATTAATAATTACAGTTGATACGGGAATATCAGCAATCCATGAAGCTAAAGTTGCAAAAGAGCTTGGT
GTCGATTTAATCATTACTGACCACCATGAGCCTGGACCGGAATTGCCAGAAGCACTAGCCATTATCCATCCAAAACTTCC
TGATAGTATTTATCCGTTTCGGGAACTCGCTGGAGTGGGAGTAGCCTTTAAATTAGCTCATGCACTATACGAAGTCATTC
CAGAGCACCTATTTGAAATCGCTGTAATTGGAACCGTTGCTGATTTAGTTTCGTTAAAGGATGAAAATCGCCTTTTAGTA
AAAAAAGGCTTAGAAAAGCTAAAGGTTACGCAAAATATAGGCCTAAAGGCAATCCTTAAATTGGCAGGTGTTAATTCACA
GGCGATAACGGAAGAAACAATTGGTTTTACGCTTGCACCGAGAATTAATGCTGTTGGCCGCCTAGAAAATGCTGATCTTG
CCGTTCAGCTGCTTCTCACTAATGATCCAAATGAAGCGCAGAGCTTAGCAGCGGAAATGGATGCTTTAAATAAACAAAGA
CAATCAATTGTTAACTCGATTACTATAGAGGCAATCGAAGAAGTGGAGAATAATTTTCCAATTGATTCGAATTCTGTCCT
TGTCATCGGTAAGGAAGGATGGAATGCAGGTGTCATTGGGATCGTTGCCTCTAGGCTAGTTGAAAAATACTACCGGCCGA
CGATTGTCCTTTCTTTTGATAAGGAAAAGGGCCTGGCAAAGGGGTCAGCACGCAGTATTGCAGGGTTTGATTTATTTGAA
AACTTATCTACATGCCGAGATATCCTGCCGCACTTCGGAGGCCATCCGATGGCTGCAGGAATGACGCTGAATCTTGAGGA
TGTCTCGTTACTGCGCTCACGATTAAATAAATTAGCAAATGAGCAATTGACAGATGAAGATTTTATTGCGGTTACATCAC
TAGACCATGAAATTACCCTTGATGAGATAAATTTACCAACACTTGAAGAATTAAACCTACTTGCACCATTTGGAATGGAC
AACCCAAAGCCGAAGGTGCTAATCAGTGATGTCCAGATTTCCTCAATCAGGAAAATTGGGTCTGATCAAAATCATTTAAA
AGTACTGGTTAATGATAGTGGTGTCAATCTAGATGGAATTGGATTTGGTTTAGGTACATTAGTTGACCAAATTTCACCAG
CCGCTAAAATATCAATAATAGGTGAATTATCTGTAAATGAGTGGAATAATATTCGTAAACCACAAATATTTATCCAAGAT
ATTGCGGTAGATACCTGGCAATTATTTGATCACCGCGGAGTGAAAAGGATTCGTTTAATGGACGGCACCATCCCGAAGGA
AAAGCGGAAATTTATTATTTTTAATAAGGAACAGCTTGATAAAGCAGATCCTGCACTAAAAGATGAGATAACATATATAC
ATGATGTTGAAGTGGCGAAAAATTTTAATAGCAAGAATGCCAATATTGTATTAATGGACTTTCCGCCTTCAAAGGAGATT
CTTATCCATTTATTTAAGGGGAAAAAACCAGCTCGGATATATGCTTATTTTCAAAAAGAGAATAGTGATTTTTTCAGCAC
GATACCGACAAGAGATCATTTTAAATGGTTTTATGCACTACTTATGAAAAAAGGCCCCATTGATTTAGGACGTTATGGGG
AAGTGATTGCAAAAAAACGAGGCTGGTCACAGGAAACGATCACCTTTATGTCACGGGTGTTTTCTGAACTGGAATTTGTT
ACAATGAACAAGGGATTTATTACGTTGAATAAACAAGCGCATAAGCGTGATTTAACTGACTCTCACACCTATCAAACGAA
ACAGGCTCAGTTTGCTCTTGAAAGAGATTTATTGTTTTCATCCTTTCAGGACCTAAAGAACTGGTTCGATGACGTCATAG
AAGAGCCGGTTATAACTGAGGAGGCAATAAAGTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-225)

DHHA1 domain (DHHA1)

(308-438)

RecJ OB domain (RecJ_OB)

(455-560)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(567-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.787

99.362

0.534

Multiple sequence alignment