Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAFX12_RS01350 Genome accession   NZ_CP151938
Coordinates   285892..286908 (+) Length   338 a.a.
NCBI ID   WP_017821733.1    Uniprot ID   A0AAW7A029
Organism   Vibrio alginolyticus strain TBV025     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 280892..291908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX12_RS01335 (AAFX12_01335) - 281323..282051 (-) 729 WP_064368816.1 redoxin family protein -
  AAFX12_RS01340 (AAFX12_01340) oxyR 282221..283132 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  AAFX12_RS01345 (AAFX12_01345) - 283186..285747 (-) 2562 WP_025768081.1 penicillin-binding protein 1A -
  AAFX12_RS01350 (AAFX12_01350) pilM 285892..286908 (+) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  AAFX12_RS01355 (AAFX12_01355) pilN 286892..287467 (+) 576 WP_064368815.1 PilN domain-containing protein Machinery gene
  AAFX12_RS01360 (AAFX12_01360) pilO 287460..288053 (+) 594 WP_017633532.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAFX12_RS01365 (AAFX12_01365) pilP 288043..288558 (+) 516 WP_064368814.1 pilus assembly protein PilP Machinery gene
  AAFX12_RS01370 (AAFX12_01370) pilQ 288584..290329 (+) 1746 WP_206949731.1 type IV pilus secretin PilQ Machinery gene
  AAFX12_RS01375 (AAFX12_01375) aroK 290517..291035 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37595.95 Da        Isoelectric Point: 7.2224

>NTDB_id=981341 AAFX12_RS01350 WP_017821733.1 285892..286908(+) (pilM) [Vibrio alginolyticus strain TBV025]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIIERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=981341 AAFX12_RS01350 WP_017821733.1 285892..286908(+) (pilM) [Vibrio alginolyticus strain TBV025]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCATAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAGGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.84

100

0.828
  pilM Vibrio cholerae strain A1552

50.448

99.112

0.5

Multiple sequence alignment