Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACLM40_RS05510 Genome accession   NZ_CP182859
Coordinates   1218402..1220618 (-) Length   738 a.a.
NCBI ID   WP_231957249.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain APPFJLYC01     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1213402..1225618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLM40_RS05490 (ACLM40_05490) - 1214508..1215278 (-) 771 WP_005597814.1 glycosyltransferase family 25 protein -
  ACLM40_RS05495 (ACLM40_05495) - 1215275..1216483 (-) 1209 WP_005597816.1 lipopolysaccharide biosynthesis protein -
  ACLM40_RS05500 (ACLM40_05500) - 1216461..1217426 (-) 966 WP_012263070.1 polysaccharide pyruvyl transferase family protein -
  ACLM40_RS05505 (ACLM40_05505) - 1217582..1218322 (+) 741 WP_418145824.1 YdcF family protein -
  ACLM40_RS05510 (ACLM40_05510) priA 1218402..1220618 (-) 2217 WP_231957249.1 primosomal protein N' Machinery gene
  ACLM40_RS05515 (ACLM40_05515) - 1220702..1221859 (-) 1158 WP_418145826.1 chorismate mutase -
  ACLM40_RS05520 (ACLM40_05520) prlC 1221982..1224015 (-) 2034 WP_418145828.1 oligopeptidase A -
  ACLM40_RS05525 (ACLM40_05525) pflA 1224158..1224895 (-) 738 WP_005597829.1 pyruvate formate lyase 1-activating protein -

Sequence

Protein


Download         Length: 738 a.a.        Molecular weight: 83230.97 Da        Isoelectric Point: 8.7569

>NTDB_id=981332 ACLM40_RS05510 WP_231957249.1 1218402..1220618(-) (priA) [Actinobacillus pleuropneumoniae strain APPFJLYC01]
MSKLVRVALPVPLHRYFDYLLPPFLSAVKGVRVEVPFGNQTKIGVAVDFPETSDVPEDKLKPIKAVLDLEPIFDEEIWTL
LRWAARYYHAPIGEVAINALPVKLRNGDSTERSQPDYFTVTEAGRAALLSGENKRAKKQQDLLAKLAEIANFFEKPTACS
QSAWKGLLEKNYIEPVNVPFELQHWQKKLQNRPLVNLHNRLVLNKQQTLVVSRLAVQNGFEAFLLNGVTGSGKTEVYLQV
IEEVLKRGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWLRAKNGESAIVIGTRSALFSQFEKLGLI
IIDEEHDSSFKQQDGWRYHARDLAILRAKNLHIPIILGSATPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQ
RITAGLSDRLLAMMKQHLEQGNQVMLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCG
HCGSTNLITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILIGTQMLAKGHHFPNVTLVAIV
NVDSALFSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQTHYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQV
LLRATGKDNQQVVNLLQNLTAYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDLDKANL
GLQNNIRLTLDIDPQEIG

Nucleotide


Download         Length: 2217 bp        

>NTDB_id=981332 ACLM40_RS05510 WP_231957249.1 1218402..1220618(-) (priA) [Actinobacillus pleuropneumoniae strain APPFJLYC01]
ATGTCGAAGCTAGTTAGAGTGGCATTACCCGTGCCGTTACATCGTTATTTTGATTATTTACTGCCGCCTTTTTTGTCGGC
GGTCAAAGGCGTGCGTGTTGAAGTGCCTTTCGGTAATCAAACGAAGATCGGCGTGGCGGTGGATTTCCCTGAAACAAGTG
ATGTGCCTGAAGATAAGCTCAAACCGATTAAAGCGGTATTAGATTTAGAACCGATCTTTGACGAAGAAATTTGGACACTG
CTTCGTTGGGCGGCTCGTTATTATCATGCGCCTATCGGCGAAGTGGCGATCAATGCTTTGCCGGTTAAATTACGTAACGG
TGATAGTACCGAAAGATCGCAACCGGATTACTTTACTGTTACTGAGGCAGGGCGGGCGGCATTGCTTTCGGGTGAGAACA
AGCGGGCCAAAAAACAGCAAGATTTACTGGCGAAACTGGCAGAAATTGCAAATTTTTTTGAAAAACCAACCGCTTGTAGT
CAATCGGCTTGGAAAGGGCTATTGGAAAAAAACTATATCGAACCGGTTAATGTGCCGTTTGAGCTGCAACATTGGCAAAA
AAAATTGCAAAATCGACCGCTTGTAAACTTGCATAATCGACTGGTATTAAATAAACAGCAAACCCTAGTGGTGAGCCGCT
TAGCAGTACAAAACGGTTTCGAAGCTTTTTTATTAAATGGCGTAACCGGTTCAGGCAAAACCGAAGTGTATTTGCAAGTG
ATCGAAGAGGTGTTAAAGCGAGGCGAGCAAGTATTGGTGTTAGTGCCGGAAATCGGGCTGACTCCGCAAACGGTGCAACG
TTTTAAAGCCCGTTTTAATGTTGAAATTGATGTTCTTCATTCGAATATGAATGAAACAGAGCGATTAAATGCTTGGTTGC
GAGCAAAAAACGGCGAAAGTGCGATTGTGATCGGCACGCGTTCGGCATTGTTCAGCCAGTTTGAGAAATTGGGCTTAATC
ATTATTGATGAAGAGCACGATAGTTCTTTCAAACAACAAGACGGCTGGCGTTATCACGCAAGAGATTTGGCAATTTTACG
GGCAAAAAATTTACATATCCCGATTATTTTGGGTTCTGCAACGCCAAGTTTGGAAAGCGTACAAAACGTTCAAAACGGTA
AGTTTATTGAGCTATCTTTAACCGCTCGTGCCGGTAATGCTCAACAGGCGAAACAGCAATTGATTGATTTAAAAACACAG
CGTATTACCGCCGGACTTTCCGACCGCTTATTAGCGATGATGAAGCAGCATTTAGAGCAAGGCAATCAGGTAATGTTGTT
TTTAAATCGCCGCGGCTTTGCACCGGTTTTACTTTGTCACGAGTGTGGCTGGATGTGTGAATGTGACGCTTGTGAAAAGC
CTTACACTTATCATCAGAAACAGAAAATCTTGCGTTGTCATCATTGTGCTTCACAGCGAGTGATCCCTCGCCAGTGCGGG
CATTGCGGCTCAACCAATCTGATTACCACCGGAGTGGGGACGGAGCAGTTGGAGCAAGTGTTAAGTGAGCAATTCCCAAC
TTACCAAATTAGCCGTATCGACCGAGATTCCACCGCTCGTAAAGGGGCATTGGAAAATCACTTAAACGATATCCGAGAAG
GCAAAAGCCAAATTTTGATTGGCACGCAAATGCTAGCGAAAGGACACCATTTTCCGAATGTAACTTTAGTGGCGATTGTG
AATGTAGATTCGGCACTGTTTTCGACCGACTTCCGTGCGGAAGAACGTTTGGCACAGCTTTATGTGCAAGTGGCGGGACG
TGCCGGACGGGCGGAAAAGCAGGGCGAAGTGGTATTACAAACTCATTATCCGGAACATCCGTTGCTCAAGACTTTGTTAG
AACAAGGTTATTTGGCGTTTGCACAAGAAGCGTTAAAAATGCGTAAAGTGATGGGCTTGCCGCCGTTTGCCGCACAAGTG
TTGTTACGGGCAACCGGCAAAGATAATCAGCAAGTGGTAAATCTTTTGCAAAATTTGACCGCTTATTTTCAGCAAAAATG
TACGGAACTCGGTTTGCAGGATATCCAGCTTCTACCGCCTTTTTCCGCACCGATGGCTAAAAAAGCGGGGCATTACCGCT
GGCTTTTATTAATTCAACATCGCTCAAGAGGCGTATTACAACAGTTGATCGATCAATTTGATTTGGATAAAGCCAATTTA
GGCTTACAAAATAATATTCGTTTAACCTTAGATATTGATCCGCAAGAAATCGGCTAA

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(497-591)

DEAD/DEAH box helicase (DEAD)

(221-369)

Primosomal protein N C-terminal domain (PriA_C)

(635-734)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(448-473)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.28

100

0.436

Multiple sequence alignment