Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAFX34_RS09620 Genome accession   NZ_CP151937
Coordinates   437332..438339 (+) Length   335 a.a.
NCBI ID   WP_011151279.1    Uniprot ID   -
Organism   Vibrio vulnificus strain TBV029     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432332..443339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX34_RS09605 (AAFX34_09595) - 433167..433439 (-) 273 WP_017789760.1 DUF3624 domain-containing protein -
  AAFX34_RS09610 (AAFX34_09600) oxyR 433713..434612 (+) 900 WP_011079350.1 DNA-binding transcriptional regulator OxyR -
  AAFX34_RS09615 (AAFX34_09605) - 434679..437192 (-) 2514 WP_103175341.1 penicillin-binding protein 1A -
  AAFX34_RS09620 (AAFX34_09610) pilM 437332..438339 (+) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  AAFX34_RS09625 (AAFX34_09615) pilN 438329..438919 (+) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  AAFX34_RS09630 (AAFX34_09620) pilO 438912..439502 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAFX34_RS09635 (AAFX34_09625) pilP 439492..440004 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  AAFX34_RS09640 (AAFX34_09630) pilQ 440018..441802 (+) 1785 WP_011079356.1 type IV pilus secretin PilQ Machinery gene
  AAFX34_RS09645 (AAFX34_09635) aroK 442004..442522 (+) 519 WP_011079357.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37638.05 Da        Isoelectric Point: 6.3873

>NTDB_id=981306 AAFX34_RS09620 WP_011151279.1 437332..438339(+) (pilM) [Vibrio vulnificus strain TBV029]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVALAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=981306 AAFX34_RS09620 WP_011151279.1 437332..438339(+) (pilM) [Vibrio vulnificus strain TBV029]
ATGGGTAAGCCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAACTGCCTCTTTTCCGTTATCAGGTGGCACTGGCGGTGCCTGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAGCGAGAATTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCGTTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGTGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGACGTGCATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGATAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCGCCTTTAGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAACTGACAGAGCGAGTTCGTCGGCAATTGCAGCTGTTCCAATCTATGGATAGCCAAAGCAAAGTGAATGGCCTCTGGAT
CTGCGGTGAAGGCGCGATTACTCCGTTGTTGAGTGAAACGTTGCAGAGAAAGCTGAGTTTGGAGTGCGAAGTCCTTAATC
CTCTGGCTCTGTTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTGGCAGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.433

100

0.534
  pilM Vibrio cholerae strain A1552

49.267

100

0.501

Multiple sequence alignment