Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   WLF14_RS02325 Genome accession   NZ_CP151816
Coordinates   513675..514997 (+) Length   440 a.a.
NCBI ID   WP_041160823.1    Uniprot ID   A0A5M8F1M7
Organism   Pseudomonas fluorescens strain IMGN2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508675..519997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WLF14_RS02305 (WLF14_02305) - 508740..511205 (-) 2466 WP_342308808.1 penicillin-binding protein 1A -
  WLF14_RS02310 (WLF14_02310) pilM 511424..512080 (+) 657 WP_102625309.1 type IV pilus assembly protein PilM -
  WLF14_RS02315 (WLF14_02315) - 512077..512628 (+) 552 WP_024072961.1 PilN domain-containing protein -
  WLF14_RS02320 (WLF14_02320) - 512625..513605 (+) 981 WP_342308809.1 pilus assembly protein PilP -
  WLF14_RS02325 (WLF14_02325) pilQ 513675..514997 (+) 1323 WP_041160823.1 type IV pilus secretin PilQ family protein Machinery gene
  WLF14_RS02330 (WLF14_02330) aroK 515002..515520 (+) 519 WP_024072964.1 shikimate kinase AroK -
  WLF14_RS02335 (WLF14_02335) aroB 515646..516746 (+) 1101 WP_024072965.1 3-dehydroquinate synthase -
  WLF14_RS02340 (WLF14_02340) - 516757..518313 (+) 1557 WP_024072966.1 AAA family ATPase -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 47903.74 Da        Isoelectric Point: 4.8958

>NTDB_id=980993 WLF14_RS02325 WP_041160823.1 513675..514997(+) (pilQ) [Pseudomonas fluorescens strain IMGN2]
MVFAVPNRVDLIQLPPPESASPSYSGDKLSLNFQNIELRAVLQHIADVAGLNLVASDEVQGAITLRLRDVPWDQALDLVL
QSKGLDKRIKAGVLLVAPADELAARELLTLESRRQMAELAPLRRELLQVNYAKAADLAKLFQSVTGHEGATDERGSVAVD
DRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGARWGGRLSHGKWGAGGISKPLASGEELPDN
PPSSPFVDLGAVTGTSGLGVAFITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSSGATSVS
FKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPFF
GDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=980993 WLF14_RS02325 WP_041160823.1 513675..514997(+) (pilQ) [Pseudomonas fluorescens strain IMGN2]
ATGGTTTTCGCTGTGCCCAATCGTGTGGACCTGATCCAGCTGCCGCCACCCGAAAGCGCGTCGCCGAGCTACTCCGGCGA
CAAGCTGAGCCTCAATTTCCAGAACATCGAGCTACGCGCGGTGCTGCAACATATCGCCGATGTGGCGGGCCTCAATCTGG
TGGCCAGCGACGAAGTGCAGGGCGCGATCACCCTGCGGCTCAGGGACGTGCCCTGGGATCAAGCCCTGGACCTGGTGTTG
CAAAGCAAGGGCTTGGACAAGCGCATCAAGGCCGGTGTCTTGCTGGTGGCGCCTGCCGACGAGCTGGCCGCCCGCGAACT
GCTGACCCTGGAGTCGCGGCGGCAGATGGCCGAACTCGCGCCACTGCGCCGCGAACTGTTACAGGTCAACTACGCCAAGG
CAGCGGACCTGGCCAAGCTGTTCCAGTCGGTGACGGGGCACGAGGGCGCGACCGATGAGCGCGGTTCGGTGGCGGTGGAT
GATCGCACCAATAACATCATCGCCTACCAGACCGGCGAGCGCTTGGAGGAGCTGCGGCGGATCGTGGCGCAGTTGGACAT
CCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAACGTTGACTATGACAAAAGCCTGGGCGCGCGTTGGG
GCGGTCGATTGAGTCACGGCAAGTGGGGCGCAGGTGGTATCAGCAAACCCTTGGCGAGCGGCGAGGAACTGCCGGATAAC
CCGCCCAGTTCGCCCTTTGTCGACCTGGGCGCGGTTACGGGCACCTCAGGGCTGGGCGTCGCGTTTATCACCGATAACCT
GCTGCTGGACTTGGAACTGACGGCCATGGAGAAGACCGGCAATGGCGAAATCGTCTCGCAACCCAAGGTAGTGACGTCCG
ACAAGGAAACCGCGCGCATCCTCAAGGGCACCGAGATCCCCTACCAGGAATCCAGCTCCAGTGGCGCCACCTCGGTGTCC
TTCAAGGAGGCCTCGCTGTCACTGGAGGTCACCCCGCAAATCACGCCTGACGACCGCGTGATCATGGAGGTCAAGGTCAC
CAAGGACGAGCCCGACTACCTGAACAAGCTCAACGACGTACCGCCGATCAAGAAAAACGAGGTGAACGCGAAGGTGCTGG
TCAAGGATGGCGAGACCATCGTCATCGGCGGGGTTTTCTCGAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTTTTC
GGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCTGAC
TCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(126-191)

Secretin and TonB N terminus short domain (STN)

(52-98)

Bacterial type II and III secretion system protein (Secretin)

(275-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M8F1M7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.396

99.318

0.709
  comQ Acinetobacter baylyi ADP1

42.07

100

0.434
  pilQ Legionella pneumophila strain ERS1305867

45.238

95.455

0.432
  pilQ Vibrio cholerae strain A1552

44.056

97.5

0.43
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.056

97.5

0.43
  pilQ Acinetobacter baumannii strain A118

40.471

100

0.43
  pilQ Acinetobacter baumannii D1279779

40.471

100

0.43
  pilQ Vibrio campbellii strain DS40M4

43.519

98.182

0.427
  pilQ Legionella pneumophila strain Lp02

44.524

95.455

0.425

Multiple sequence alignment