Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAEY27_RS02625 Genome accession   NZ_CP151800
Coordinates   586575..588008 (-) Length   477 a.a.
NCBI ID   WP_342323376.1    Uniprot ID   -
Organism   Kosakonia sp. BYX6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 581575..593008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEY27_RS02595 (AAEY27_02595) elbB 583142..583795 (+) 654 WP_342323370.1 isoprenoid biosynthesis glyoxalase ElbB -
  AAEY27_RS02600 (AAEY27_02600) mtgA 583792..584523 (+) 732 WP_342323371.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AAEY27_RS02605 (AAEY27_02605) npr 584524..584796 (-) 273 WP_342323372.1 PTS phosphocarrier protein NPr -
  AAEY27_RS02610 (AAEY27_02610) rapZ 584793..585647 (-) 855 WP_342323373.1 RNase adapter RapZ -
  AAEY27_RS02615 (AAEY27_02615) ptsN 585693..586184 (-) 492 WP_342323374.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAEY27_RS02620 (AAEY27_02620) hpf 586265..586552 (-) 288 WP_342323375.1 ribosome hibernation promoting factor -
  AAEY27_RS02625 (AAEY27_02625) rpoN/rpoN1 586575..588008 (-) 1434 WP_342323376.1 RNA polymerase factor sigma-54 Machinery gene
  AAEY27_RS02630 (AAEY27_02630) lptB 588057..588782 (-) 726 WP_342323377.1 LPS export ABC transporter ATP-binding protein -
  AAEY27_RS02635 (AAEY27_02635) lptA 588789..589349 (-) 561 WP_342323378.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AAEY27_RS02640 (AAEY27_02640) lptC 589318..589893 (-) 576 WP_342323379.1 LPS export ABC transporter periplasmic protein LptC -
  AAEY27_RS02645 (AAEY27_02645) kdsC 589890..590456 (-) 567 WP_342323380.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AAEY27_RS02650 (AAEY27_02650) kdsD 590471..591457 (-) 987 WP_342323381.1 arabinose-5-phosphate isomerase KdsD -
  AAEY27_RS02655 (AAEY27_02655) - 591471..592445 (-) 975 WP_342323382.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53800.61 Da        Isoelectric Point: 4.3679

>NTDB_id=980905 AAEY27_RS02625 WP_342323376.1 586575..588008(-) (rpoN/rpoN1) [Kosakonia sp. BYX6]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVDNQQTQENEGLDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGSSSDYIDDELPIYQGETTQSLQDYLMWQVQLTPFSDTDRAIATSIVDAVDDTGYLTVSLEE
VLESMGNEDVGLDEVEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFAKDTPWCEEARLIICDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQINQQYAAMGSGARNDADTQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=980905 AAEY27_RS02625 WP_342323376.1 586575..588008(-) (rpoN/rpoN1) [Kosakonia sp. BYX6]
ATGAAGCAAGGTTTGCAACTAAGGCTTAGCCAACAGCTAGCCATGACGCCACAACTGCAACAGGCCATCCGTCTGTTGCA
GTTGTCCACGTTGGAACTTCAGCAGGAACTTCAGCAAGCGCTGGAGAGTAATCCGCTGCTGGAACAAACCGACCTTCATG
AAGAAGTGGATAACCAACAAACCCAGGAAAACGAAGGCCTGGACACCGTCGACGCACTCGAACAGAAAGAGATGCCCGAC
GAGCTGCCGCTGGACGCCAGCTGGGATGAGATTTACACCGCAGGCACCCCTTCCGGCAGCAGCAGTGATTATATTGATGA
CGAGCTGCCGATTTACCAAGGCGAAACCACGCAATCTCTGCAAGATTATCTGATGTGGCAGGTGCAACTGACGCCGTTTT
CCGATACTGACCGCGCTATCGCGACCTCCATTGTTGATGCCGTTGACGATACCGGTTATCTCACCGTGTCGCTGGAAGAG
GTGCTGGAAAGTATGGGCAACGAAGACGTCGGTCTTGATGAAGTGGAAGCGGTGCTCAAGCGCATCCAACGCTTCGACCC
GGTCGGCGTGGCGGCGCGGGATCTGCGCGACTGCCTGTTAGTGCAACTTTCCCAGTTCGCGAAAGACACGCCGTGGTGTG
AAGAAGCCCGGCTTATCATCTGCGATCATCTGGATCTGCTCGCCAATCACGATTTCCGCACGTTAATGCGCGTCACGCGC
CTGAAAGAAGATGTGTTGAAAGAAGCGGTCAATCTGATCCAATCTCTGGATCCGCGCCCTGGTCAGTCGATCCAAACTGG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGCGCAAGCATAACGGGCGCTGGACCGTGGAGTTAAACTCCGACAGTA
TCCCGCGTCTGCAAATCAACCAGCAATATGCCGCAATGGGCAGTGGCGCGCGTAATGACGCCGACACGCAGTTCATTCGC
AGTAACTTGCAGGAAGCGAAGTGGTTGATCAAAAGCCTGGAAAGCCGCAATGACACCCTGTTACGCGTCAGTCGCTGCAT
TGTCGAGCAGCAACAAGCGTTCTTTGAGCAGGGTGAAGAATTTATGAAGCCGATGGTGCTGGCAGATATCGCCCAAGCCG
TCGAGATGCACGAATCCACCATTTCCCGTGTGACGACGCAGAAGTACCTGCACAGCCCTCGCGGCATTTTTGAATTGAAG
TATTTCTTCTCCAGCCATGTGAATACCGAAGGCGGCGGCGAAGCTTCGTCCACGGCTATTCGCGCACTGGTGAAGAAATT
GATCGCCGCAGAAAACCCTGCAAAACCACTGAGCGACAGTAAGCTGACGACCATACTTTCCGATCAGGGAATTATGGTGG
CGCGGCGCACCGTCGCGAAGTATCGAGAATCTTTATCCATTCCGCCGTCAAATCAGCGCAAACAGTTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.136

100

0.486

Multiple sequence alignment