Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACNJZ5_RS04770 Genome accession   NZ_CP182371
Coordinates   1024502..1025998 (+) Length   498 a.a.
NCBI ID   WP_001551783.1    Uniprot ID   -
Organism   Escherichia coli strain Br_112_kylling     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1019502..1030998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNJZ5_RS04740 tusA 1019749..1019994 (+) 246 WP_000130615.1 sulfurtransferase TusA -
  ACNJZ5_RS04745 zntA 1020096..1022294 (-) 2199 WP_001551784.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  ACNJZ5_RS04750 yhhN 1022368..1022994 (-) 627 WP_000964718.1 lysoplasmalogenase -
  ACNJZ5_RS04755 yhhM 1023135..1023494 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  ACNJZ5_RS04760 yhhL 1023497..1023766 (-) 270 WP_001295207.1 DUF1145 family protein -
  ACNJZ5_RS04765 rsmD 1023756..1024352 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  ACNJZ5_RS04770 pilA 1024502..1025998 (+) 1497 WP_001551783.1 signal recognition particle-docking protein FtsY Machinery gene
  ACNJZ5_RS04775 ftsE 1026001..1026669 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  ACNJZ5_RS04780 ftsX 1026662..1027720 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  ACNJZ5_RS04785 rpoH 1027965..1028819 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  ACNJZ5_RS04790 livJ 1029090..1030193 (+) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  ACNJZ5_RS04795 panM 1030393..1030776 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54701.53 Da        Isoelectric Point: 4.1660

>NTDB_id=980550 ACNJZ5_RS04770 WP_001551783.1 1024502..1025998(+) (pilA) [Escherichia coli strain Br_112_kylling]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAE
SEKAQPEAEVVAQPELVAEETPEPVAIEREELPLPEDVNAEEVSPEEWRAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=980550 ACNJZ5_RS04770 WP_001551783.1 1024502..1025998(+) (pilA) [Escherichia coli strain Br_112_kylling]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAAGCGGAAACTTTTGCTGCCGAAGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACTGGTCGCAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCGGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCAGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTAGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACTAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATTCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.864

74.096

0.369

Multiple sequence alignment