Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACM2XU_RS02700 Genome accession   NZ_CP182182
Coordinates   573671..575104 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain EC19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 568671..580104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM2XU_RS02665 (ACM2XU_02665) elbB 569363..570016 (+) 654 WP_001302019.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACM2XU_RS02670 (ACM2XU_02670) mtgA 570013..570741 (+) 729 WP_000047098.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACM2XU_RS02675 (ACM2XU_02675) yrbL 570738..571370 (-) 633 WP_000620399.1 PhoP regulatory network protein YrbL -
  ACM2XU_RS02680 (ACM2XU_02680) npr 571583..571855 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACM2XU_RS02685 (ACM2XU_02685) rapZ 571852..572706 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACM2XU_RS02690 (ACM2XU_02690) ptsN 572752..573243 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACM2XU_RS02695 (ACM2XU_02695) hpf 573361..573648 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACM2XU_RS02700 (ACM2XU_02700) rpoN/rpoN1 573671..575104 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACM2XU_RS02705 (ACM2XU_02705) lptB 575152..575877 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACM2XU_RS02710 (ACM2XU_02710) lptA 575884..576441 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACM2XU_RS02715 (ACM2XU_02715) lptC 576410..576985 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACM2XU_RS02720 (ACM2XU_02720) kdsC 576982..577548 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACM2XU_RS02725 (ACM2XU_02725) kdsD 577569..578555 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACM2XU_RS02730 (ACM2XU_02730) yrbG 578569..579546 (-) 978 WP_022646167.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=979963 ACM2XU_RS02700 WP_000809051.1 573671..575104(-) (rpoN/rpoN1) [Escherichia coli strain EC19]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=979963 ACM2XU_RS02700 WP_000809051.1 573671..575104(-) (rpoN/rpoN1) [Escherichia coli strain EC19]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACTGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465

Multiple sequence alignment