Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R8566_RS10445 Genome accession   NZ_AP026920
Coordinates   2000128..2001069 (-) Length   313 a.a.
NCBI ID   WP_000249564.1    Uniprot ID   Q8DN86
Organism   Streptococcus pneumoniae strain PZ900700014     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1995128..2006069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8566_RS10405 (PC0014_20500) comYH 1995621..1996574 (-) 954 WP_000345085.1 class I SAM-dependent methyltransferase Machinery gene
  R8566_RS10410 - 1996635..1997222 (-) 588 Protein_2021 class I SAM-dependent methyltransferase -
  R8566_RS10415 (PC0014_20530) comGG/cglG 1997359..1997772 (-) 414 WP_000265619.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8566_RS10420 (PC0014_20540) comGF/cglF 1997750..1998211 (-) 462 WP_000250523.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8566_RS10425 (PC0014_20550) comGE/cglE 1998174..1998476 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8566_RS10430 (PC0014_20560) comGD/cglD 1998439..1998843 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8566_RS10435 (PC0014_20570) comGC/cglC 1998836..1999162 (-) 327 WP_000738638.1 comG operon protein ComGC Machinery gene
  R8566_RS10440 (PC0014_20580) comGB/cglB 1999164..2000180 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8566_RS10445 (PC0014_20590) comGA/cglA/cilD 2000128..2001069 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  R8566_RS10450 (PC0014_20600) - 2001145..2001510 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8566_RS10455 (PC0014_20610) - 2001661..2002719 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R8566_RS10460 (PC0014_20620) nagA 2002882..2004033 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R8566_RS10465 (PC0014_20630) - 2004186..2006003 (-) 1818 WP_001220853.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35555.47 Da        Isoelectric Point: 6.1330

>NTDB_id=97989 R8566_RS10445 WP_000249564.1 2000128..2001069(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700014]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=97989 R8566_RS10445 WP_000249564.1 2000128..2001069(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700014]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTATAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DN86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae D39

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae R6

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

100

100

1
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment