Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R8566_RS10440 Genome accession   NZ_AP026920
Coordinates   1999164..2000180 (-) Length   338 a.a.
NCBI ID   WP_074196785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700014     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1994164..2005180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8566_RS10400 (PC0014_20490) - 1994380..1995570 (-) 1191 WP_000167766.1 acetate kinase -
  R8566_RS10405 (PC0014_20500) comYH 1995621..1996574 (-) 954 WP_000345085.1 class I SAM-dependent methyltransferase Machinery gene
  R8566_RS10410 - 1996635..1997222 (-) 588 Protein_2021 class I SAM-dependent methyltransferase -
  R8566_RS10415 (PC0014_20530) comGG/cglG 1997359..1997772 (-) 414 WP_000265619.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8566_RS10420 (PC0014_20540) comGF/cglF 1997750..1998211 (-) 462 WP_000250523.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8566_RS10425 (PC0014_20550) comGE/cglE 1998174..1998476 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8566_RS10430 (PC0014_20560) comGD/cglD 1998439..1998843 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8566_RS10435 (PC0014_20570) comGC/cglC 1998836..1999162 (-) 327 WP_000738638.1 comG operon protein ComGC Machinery gene
  R8566_RS10440 (PC0014_20580) comGB/cglB 1999164..2000180 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8566_RS10445 (PC0014_20590) comGA/cglA/cilD 2000128..2001069 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  R8566_RS10450 (PC0014_20600) - 2001145..2001510 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8566_RS10455 (PC0014_20610) - 2001661..2002719 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R8566_RS10460 (PC0014_20620) nagA 2002882..2004033 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38392.40 Da        Isoelectric Point: 9.4802

>NTDB_id=97988 R8566_RS10440 WP_074196785.1 1999164..2000180(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700014]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=97988 R8566_RS10440 WP_074196785.1 1999164..2000180(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700014]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

100

100

1
  comGB/cglB Streptococcus pneumoniae D39

100

100

1
  comGB/cglB Streptococcus pneumoniae R6

100

100

1
  comGB/cglB Streptococcus pneumoniae TIGR4

100

100

1
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.547

94.083

0.541
  comYB Streptococcus mutans UA159

57.547

94.083

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment