Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   R8566_RS10420 Genome accession   NZ_AP026920
Coordinates   1997750..1998211 (-) Length   153 a.a.
NCBI ID   WP_000250523.1    Uniprot ID   A0A0B7M7T3
Organism   Streptococcus pneumoniae strain PZ900700014     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1992750..2003211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8566_RS10390 (PC0014_20470) - 1993062..1993886 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  R8566_RS10395 (PC0014_20480) rnpA 1993861..1994232 (-) 372 WP_000739241.1 ribonuclease P protein component -
  R8566_RS10400 (PC0014_20490) - 1994380..1995570 (-) 1191 WP_000167766.1 acetate kinase -
  R8566_RS10405 (PC0014_20500) comYH 1995621..1996574 (-) 954 WP_000345085.1 class I SAM-dependent methyltransferase Machinery gene
  R8566_RS10410 - 1996635..1997222 (-) 588 Protein_2021 class I SAM-dependent methyltransferase -
  R8566_RS10415 (PC0014_20530) comGG/cglG 1997359..1997772 (-) 414 WP_000265619.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8566_RS10420 (PC0014_20540) comGF/cglF 1997750..1998211 (-) 462 WP_000250523.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8566_RS10425 (PC0014_20550) comGE/cglE 1998174..1998476 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8566_RS10430 (PC0014_20560) comGD/cglD 1998439..1998843 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8566_RS10435 (PC0014_20570) comGC/cglC 1998836..1999162 (-) 327 WP_000738638.1 comG operon protein ComGC Machinery gene
  R8566_RS10440 (PC0014_20580) comGB/cglB 1999164..2000180 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8566_RS10445 (PC0014_20590) comGA/cglA/cilD 2000128..2001069 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  R8566_RS10450 (PC0014_20600) - 2001145..2001510 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8566_RS10455 (PC0014_20610) - 2001661..2002719 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17821.32 Da        Isoelectric Point: 6.4832

>NTDB_id=97984 R8566_RS10420 WP_000250523.1 1997750..1998211(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700014]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=97984 R8566_RS10420 WP_000250523.1 1997750..1998211(-) (comGF/cglF) [Streptococcus pneumoniae strain PZ900700014]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M7T3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae D39

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae R6

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae TIGR4

94.771

100

0.948
  comGF/cglF Streptococcus mitis SK321

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

92.157

100

0.922

Multiple sequence alignment