Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AAE485_RS02045 Genome accession   NZ_CP151686
Coordinates   397742..399100 (-) Length   452 a.a.
NCBI ID   WP_215893283.1    Uniprot ID   -
Organism   Acidithiobacillus ferriphilus strain QBS3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 392742..404100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAE485_RS02025 (AAE485_02000) - 394456..394761 (-) 306 WP_064219185.1 Lpp/OprI family alanine-zipper lipoprotein -
  AAE485_RS02030 (AAE485_02005) - 395164..395736 (+) 573 WP_215860509.1 hypothetical protein -
  AAE485_RS02035 (AAE485_02010) pepP 395772..397118 (-) 1347 WP_215849222.1 Xaa-Pro aminopeptidase -
  AAE485_RS02040 (AAE485_02015) - 397134..397571 (-) 438 WP_409409372.1 DUF4168 domain-containing protein -
  AAE485_RS02045 (AAE485_02020) radA 397742..399100 (-) 1359 WP_215893283.1 DNA repair protein RadA Machinery gene
  AAE485_RS02050 (AAE485_02025) alr 399097..400191 (-) 1095 WP_409409373.1 alanine racemase -
  AAE485_RS02055 (AAE485_02030) dnaB 400254..401627 (-) 1374 WP_409409374.1 replicative DNA helicase -
  AAE485_RS02060 (AAE485_02035) rplI 401653..402099 (-) 447 WP_064219192.1 50S ribosomal protein L9 -
  AAE485_RS02065 (AAE485_02040) - 402137..403054 (-) 918 WP_215893286.1 DUF2232 domain-containing protein -
  AAE485_RS02070 (AAE485_02045) rpsR 403070..403348 (-) 279 WP_014027827.1 30S ribosomal protein S18 -
  AAE485_RS02075 (AAE485_02050) rpsF 403364..403768 (-) 405 WP_064219194.1 30S ribosomal protein S6 -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 47898.82 Da        Isoelectric Point: 8.0841

>NTDB_id=979814 AAE485_RS02045 WP_215893283.1 397742..399100(-) (radA) [Acidithiobacillus ferriphilus strain QBS3]
MSRDKTLFVCQECGSTSNKWLGRCPDCGAWNSFVEQRMEKTGAKSQTTYATASPPQFLHAVPIADVGRSTTGLAELDRVL
GGGQVPGAAILLGGEPGIGKSTLLLQTAHHLAQASKVLYVTGEESAAQVALRAQRLGLGQSSVRVVAENHLEAIEGLLQS
ERSALVLVDSIQTVYTDTLQSAPGSVAQVRECAARLVRFAKATDTTVWLVGHVTKEGAIAGPRVLEHMVDTVLYFEGEAG
SPYRIVRAIKNRFGAANELGVFQMHEEGLSEVANPSQLFLSQHDRPVAGSVVLATQEGTRPLLVEVQALVTPSPLANPRR
VAIGLDPNRLSLLLAILHRHGGSVFFDQDVFVNIAGGIKVNEPAADLAVALALLSSFRNKPLEGRRVVFGELGLAGEVRA
VAGTEARVREAIKLGFKGAILPRGDKISSTATFKLRPAARLGDAITAAFDGG

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=979814 AAE485_RS02045 WP_215893283.1 397742..399100(-) (radA) [Acidithiobacillus ferriphilus strain QBS3]
ATGAGCCGCGACAAAACCCTCTTTGTCTGTCAGGAATGCGGCAGTACCAGCAACAAATGGCTGGGACGCTGCCCGGATTG
TGGCGCCTGGAACAGCTTCGTCGAACAGCGCATGGAGAAGACCGGGGCCAAATCCCAAACCACCTATGCCACGGCCAGTC
CGCCGCAATTTCTGCACGCGGTGCCGATCGCGGATGTGGGGCGCAGCACGACGGGTCTGGCAGAGCTCGACCGGGTGCTG
GGCGGTGGGCAGGTGCCGGGGGCCGCCATTCTCCTGGGCGGGGAACCGGGTATCGGCAAATCCACCCTGCTGCTCCAGAC
CGCCCATCATCTGGCCCAGGCCAGTAAGGTGCTTTACGTCACCGGCGAGGAGTCCGCGGCACAAGTCGCCTTGCGCGCGC
AGCGCTTGGGGCTGGGCCAGAGCTCCGTACGGGTAGTCGCCGAAAACCATCTTGAAGCGATCGAGGGTCTGCTCCAGTCA
GAGCGATCCGCACTGGTGCTGGTGGATTCTATCCAGACCGTCTATACCGACACGCTGCAATCAGCCCCTGGATCGGTAGC
GCAAGTGCGGGAGTGCGCGGCCCGTCTGGTGCGTTTCGCCAAAGCTACGGACACCACGGTCTGGCTGGTCGGCCATGTGA
CCAAGGAGGGGGCCATTGCCGGCCCGCGTGTGCTGGAACACATGGTGGATACGGTCCTTTATTTTGAAGGGGAGGCGGGT
AGCCCCTACCGGATTGTGCGTGCCATCAAAAATCGCTTTGGTGCCGCCAATGAACTAGGTGTTTTTCAGATGCATGAAGA
GGGGTTAAGCGAGGTTGCCAACCCTTCACAGCTCTTTCTGTCGCAGCATGACCGACCCGTAGCAGGGAGCGTGGTGCTGG
CGACGCAGGAGGGAACCCGCCCGCTTCTGGTGGAAGTGCAGGCTCTGGTGACACCGAGTCCGCTGGCTAACCCCCGCAGG
GTCGCCATCGGCCTGGACCCTAACCGCCTTTCCCTCCTGCTGGCTATCCTGCACCGCCACGGCGGCAGTGTGTTTTTTGA
TCAGGACGTTTTTGTGAATATCGCAGGTGGAATCAAAGTCAACGAACCAGCCGCCGATCTTGCGGTGGCCTTGGCCCTGC
TCAGCAGTTTCCGCAACAAACCTTTGGAGGGGCGCCGGGTTGTTTTCGGCGAGCTGGGTCTGGCGGGAGAGGTGCGTGCG
GTGGCGGGCACTGAAGCGCGGGTGCGGGAGGCGATCAAATTGGGTTTTAAGGGTGCCATTCTTCCGCGTGGCGACAAGAT
TTCGAGTACCGCTACTTTCAAGTTGCGCCCGGCTGCCCGTCTTGGCGATGCCATAACAGCGGCCTTCGACGGGGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-423)

AAA domain (AAA_25)

(65-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.82

94.469

0.48
  radA Streptococcus pneumoniae Rx1

48.148

95.575

0.46
  radA Streptococcus pneumoniae D39

48.148

95.575

0.46
  radA Streptococcus pneumoniae R6

48.148

95.575

0.46
  radA Streptococcus pneumoniae TIGR4

48.148

95.575

0.46
  radA Streptococcus mitis SK321

48.148

95.575

0.46
  radA Streptococcus mitis NCTC 12261

48.148

95.575

0.46

Multiple sequence alignment