Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACNHKQ_RS20270 Genome accession   NZ_CP182139
Coordinates   4136331..4137764 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain CUWW06     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4131331..4142764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNHKQ_RS20240 (ACNHKQ_20240) yrbG 4131889..4132866 (+) 978 WP_000922872.1 calcium/sodium antiporter -
  ACNHKQ_RS20245 (ACNHKQ_20245) kdsD 4132880..4133866 (+) 987 WP_032281662.1 arabinose-5-phosphate isomerase KdsD -
  ACNHKQ_RS20250 (ACNHKQ_20250) kdsC 4133887..4134453 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACNHKQ_RS20255 (ACNHKQ_20255) lptC 4134450..4135025 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACNHKQ_RS20260 (ACNHKQ_20260) lptA 4134994..4135551 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACNHKQ_RS20265 (ACNHKQ_20265) lptB 4135558..4136283 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACNHKQ_RS20270 (ACNHKQ_20270) rpoN/rpoN1 4136331..4137764 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACNHKQ_RS20275 (ACNHKQ_20275) hpf 4137787..4138074 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACNHKQ_RS20280 (ACNHKQ_20280) ptsN 4138192..4138683 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACNHKQ_RS20285 (ACNHKQ_20285) rapZ 4138729..4139583 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ACNHKQ_RS20290 (ACNHKQ_20290) npr 4139580..4139852 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACNHKQ_RS20295 (ACNHKQ_20295) yrbL 4140065..4140697 (+) 633 WP_000620410.1 PhoP regulatory network protein YrbL -
  ACNHKQ_RS20300 (ACNHKQ_20300) mtgA 4140694..4141422 (-) 729 WP_000047087.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACNHKQ_RS20305 (ACNHKQ_20305) elbB 4141419..4142072 (-) 654 WP_001308080.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=979539 ACNHKQ_RS20270 WP_000809051.1 4136331..4137764(+) (rpoN/rpoN1) [Escherichia coli strain CUWW06]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=979539 ACNHKQ_RS20270 WP_000809051.1 4136331..4137764(+) (rpoN/rpoN1) [Escherichia coli strain CUWW06]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTACCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGACAATCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCGGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465