Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   R8613_RS09725 Genome accession   NZ_AP026919
Coordinates   1864790..1865614 (-) Length   274 a.a.
NCBI ID   WP_001036786.1    Uniprot ID   A0A0B7LDF1
Organism   Streptococcus pneumoniae strain PZ900700012     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1859790..1870614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8613_RS09720 (PC0012_18860) - 1863168..1864499 (-) 1332 WP_000390042.1 hemolysin family protein -
  R8613_RS09725 (PC0012_18870) endA 1864790..1865614 (-) 825 WP_001036786.1 DNA-entry nuclease EndA Machinery gene
  R8613_RS09730 (PC0012_18880) - 1865653..1865841 (-) 189 WP_001813390.1 DNA-directed RNA polymerase subunit beta -
  R8613_RS09735 (PC0012_18890) murA 1865834..1867117 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  R8613_RS09740 (PC0012_18900) - 1867141..1867371 (-) 231 WP_000250392.1 DUF1146 family protein -
  R8613_RS09745 (PC0012_18910) sepM 1867418..1868467 (-) 1050 WP_317648238.1 SepM family pheromone-processing serine protease Regulator
  R8613_RS09750 (PC0012_18920) coaD 1868451..1868939 (-) 489 WP_001280745.1 pantetheine-phosphate adenylyltransferase -
  R8613_RS09755 (PC0012_18930) rsmD 1868929..1869468 (-) 540 WP_000706960.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  R8613_RS09760 (PC0012_18940) asnA 1869533..1870525 (-) 993 WP_000747990.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29921.59 Da        Isoelectric Point: 10.1197

>NTDB_id=97899 R8613_RS09725 WP_001036786.1 1864790..1865614(-) (endA) [Streptococcus pneumoniae strain PZ900700012]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=97899 R8613_RS09725 WP_001036786.1 1864790..1865614(-) (endA) [Streptococcus pneumoniae strain PZ900700012]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7LDF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

99.635

100

0.996
  endA Streptococcus pneumoniae D39

99.635

100

0.996
  endA Streptococcus pneumoniae R6

99.635

100

0.996
  endA Streptococcus pneumoniae TIGR4

99.635

100

0.996

Multiple sequence alignment