Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AADV39_RS12165 Genome accession   NZ_CP151288
Coordinates   2518509..2519123 (-) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain M4019     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2513509..2524123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADV39_RS12135 rpsT 2513812..2514078 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  AADV39_RS12140 holA 2514095..2515136 (-) 1042 Protein_2343 DNA polymerase III subunit delta -
  AADV39_RS12145 - 2515176..2515327 (-) 152 Protein_2344 hypothetical protein -
  AADV39_RS12150 - 2515368..2515502 (+) 135 WP_003152870.1 YqzM family protein -
  AADV39_RS12155 comEC 2515521..2517872 (-) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AADV39_RS12160 - 2517873..2518442 (-) 570 WP_003152868.1 ComE operon protein 2 -
  AADV39_RS12165 comEA 2518509..2519123 (-) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  AADV39_RS12170 comER 2519182..2520003 (+) 822 WP_012118027.1 late competence protein ComER -
  AADV39_RS12175 - 2520072..2520809 (-) 738 WP_032873951.1 class I SAM-dependent methyltransferase -
  AADV39_RS12180 rsfS 2520806..2521162 (-) 357 WP_007408260.1 ribosome silencing factor -
  AADV39_RS12185 yqeK 2521166..2521741 (-) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AADV39_RS12190 - 2521731..2522300 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  AADV39_RS12195 yhbY 2522311..2522601 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  AADV39_RS12200 aroE 2522595..2523437 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=976137 AADV39_RS12165 WP_032873955.1 2518509..2519123(-) (comEA) [Bacillus velezensis strain M4019]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=976137 AADV39_RS12165 WP_032873955.1 2518509..2519123(-) (comEA) [Bacillus velezensis strain M4019]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363

Multiple sequence alignment