Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   ACK2OW_RS13320 Genome accession   NZ_CP180575
Coordinates   2484200..2486029 (+) Length   609 a.a.
NCBI ID   WP_003245178.1    Uniprot ID   -
Organism   Bacillus subtilis strain K3C     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2479200..2491029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2OW_RS13285 (ACK2OW_13285) yjbC 2479869..2480447 (+) 579 WP_003224597.1 GNAT family N-acetyltransferase -
  ACK2OW_RS13290 (ACK2OW_13290) spx 2480628..2481023 (+) 396 WP_014476435.1 transcriptional regulator Spx -
  ACK2OW_RS13295 (ACK2OW_13295) yjbE 2481069..2481725 (-) 657 WP_003232944.1 TerC family protein -
  ACK2OW_RS13300 (ACK2OW_13300) - 2481895..2482035 (+) 141 WP_163138320.1 hypothetical protein -
  ACK2OW_RS13305 (ACK2OW_13305) mecA 2482002..2482658 (+) 657 WP_003232942.1 adaptor protein MecA Regulator
  ACK2OW_RS13310 (ACK2OW_13310) - 2482653..2482775 (-) 123 WP_161621487.1 hypothetical protein -
  ACK2OW_RS13315 (ACK2OW_13315) coiA 2482819..2483970 (+) 1152 WP_411225631.1 competence protein CoiA Machinery gene
  ACK2OW_RS13320 (ACK2OW_13320) pepF 2484200..2486029 (+) 1830 WP_003245178.1 oligoendopeptidase F Regulator
  ACK2OW_RS13325 (ACK2OW_13325) - 2486067..2486234 (-) 168 WP_003244944.1 hypothetical protein -
  ACK2OW_RS13330 (ACK2OW_13330) spxH 2486549..2487448 (-) 900 WP_003245184.1 protease adaptor protein SpxH -
  ACK2OW_RS13335 (ACK2OW_13335) bhbI 2487445..2487843 (-) 399 WP_032725404.1 thiol management oxidoreductase -
  ACK2OW_RS13340 (ACK2OW_13340) cwlQ 2488098..2488832 (-) 735 WP_411225633.1 bifunctional muramidase/murein lytic transglycosylase -
  ACK2OW_RS13345 (ACK2OW_13345) yjbK 2488847..2489419 (-) 573 WP_015252351.1 CYTH domain-containing protein -
  ACK2OW_RS13350 (ACK2OW_13350) - 2489544..2489912 (+) 369 WP_021479543.1 hypothetical protein -
  ACK2OW_RS13355 (ACK2OW_13355) yjbM 2489941..2490576 (+) 636 WP_003245294.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70085.95 Da        Isoelectric Point: 5.1562

>NTDB_id=975034 ACK2OW_RS13320 WP_003245178.1 2484200..2486029(+) (pepF) [Bacillus subtilis strain K3C]
MAEEKKANQLPDRSEVKAEDTWRLEDIFPSDEAWNKEFQAVKELIPNLSKYKGKLADSADHLYEALTYQDKVMERLGRLY
TYAHMRSDQDTGNSFYQGLNDKAGNLYTQAASATAYLVPEILSIEEDKLQQFILEKEELKLYSHAIEEITKERPHVLSEK
EEALLAEASEVLGSSSNTFSVLNNADITFPSIKDEDGNEKQITHGNFINFLESENREVRKNAFDAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLTNVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFL
KAGSSQYPIDVLKKAGVDMTSPEPIEAACKMFEEKLDEMEELLMKVKQS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=975034 ACK2OW_RS13320 WP_003245178.1 2484200..2486029(+) (pepF) [Bacillus subtilis strain K3C]
ATGGCTGAGGAAAAAAAAGCAAACCAACTGCCTGACAGAAGTGAGGTAAAGGCAGAAGACACATGGAGACTTGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCTGTAAAAGAATTAATTCCGAATTTATCTAAGTATAAAGGAA
AGCTGGCAGATTCAGCTGATCATTTATACGAGGCTCTTACGTATCAAGATAAAGTGATGGAGCGGCTGGGTAGGCTGTAC
ACATATGCGCATATGCGCTCAGACCAGGATACTGGGAACTCCTTTTACCAGGGGCTGAATGACAAGGCGGGAAACCTGTA
TACACAGGCCGCAAGCGCGACAGCTTATTTGGTTCCGGAGATTTTATCCATAGAAGAAGACAAACTGCAGCAGTTCATTC
TTGAAAAAGAAGAATTAAAGCTGTACTCTCATGCGATTGAGGAGATTACAAAGGAACGTCCGCATGTGCTGAGCGAGAAG
GAAGAGGCGCTGCTTGCTGAAGCCTCCGAGGTACTGGGATCATCTTCAAATACATTCAGCGTGTTAAATAACGCAGATAT
AACGTTTCCGTCCATTAAAGACGAAGATGGGAATGAAAAACAGATCACTCACGGCAACTTTATTAATTTCTTGGAAAGTG
AAAACCGTGAAGTCCGCAAAAATGCGTTTGACGCAGTGTATAAAACGTACGGACAATATAAAAACACAATGGCCACGACG
CTAAGCGGCACTGTGAAAAAGGACAACTTCTACGCGAGAGTGAAAAAGTACAAGTCCGCGCGTGAGGCTGCGCTTTCTAA
CAACAGTATTCCAGAGGAAGTATACGATAACCTTGTTAAGACGATTAATAAGCATTTGCCGCTCCTGCACCGCTATATTG
CGCTAAGAAAGAAAGTGCTTGAGCTTGATGAAGTGCATATCTATGACCTGTATACACCGCTTGTGAAAGATGCTGGGATG
AAGGTGACATACGAGGAAGCCAAAGATTACATGCTGAAAGGCCTTGCACCTTTAGGGGAAGAATATGCCTCTATCCTAAA
AGAAGGACTGGAAAACCGCTGGGTGGACGTTTACGAGAATAAAGGCAAACGCAATGGGGCTTATTCATCAGGAGCTTACG
GCACGAATCCGTATATTTTGATGAACTGGCATAATAACGTCAATAATCTCTTTACGCTCGTGCACGAGTTTGGACATTCC
GTACACAGCTACTATACGAGAAAGCACCAGCCTTATCCATACGGCAATTACAGTATCTTTGTCGCGGAAGTTGCCTCTAC
GACAAATGAAGCTCTCCTTGGCGAATATTTGCTGAACAATTTAGAGGATGAAAAACAGCGCTTATATATTCTCAACCATA
TGCTTGAGGGCTTCAGGGGAACGGTCTTCAGACAAACGATGTTCGCTGAATTTGAACATCTGATCCATACAAAGGCGCAA
GAAGGCGAGCCGCTTACACCTGAGCTTCTGACAAATGTCTATTACGACCTGAATAAAAAGTATTTTGGAGACGGCATGGT
GATTGATAAGGAAATCGGCCTTGAATGGTCGCGTATTCCGCACTTCTATTACAATTACTATGTGTATCAGTATGCGACAG
GGTACAGCGCTGCCCAAGCATTAAGCAGCCAGATTTTGAAGGAAGGAAAGCCGGCGGTTGACCGTTATATTGACTTCCTG
AAAGCGGGAAGCTCACAATACCCGATTGATGTCCTGAAAAAAGCGGGTGTTGATATGACGTCTCCAGAGCCAATCGAAGC
CGCGTGCAAAATGTTTGAAGAAAAACTTGATGAAATGGAAGAGCTGCTGATGAAAGTCAAGCAGTCTTAA

Domains

Predicted by InterProScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.822

97.537

0.476

Multiple sequence alignment