Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   ACK2OW_RS03895 Genome accession   NZ_CP180575
Coordinates   663423..663833 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain K3C     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 658423..668833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2OW_RS03865 (ACK2OW_03865) - 658509..658649 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ACK2OW_RS03870 (ACK2OW_03870) yqeF 658955..659686 (-) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  ACK2OW_RS03875 (ACK2OW_03875) cwlH 659938..660690 (-) 753 WP_416042547.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ACK2OW_RS03880 (ACK2OW_03880) yqeD 660877..661503 (+) 627 WP_120028373.1 TVP38/TMEM64 family protein -
  ACK2OW_RS03885 (ACK2OW_03885) gnd 661523..662416 (-) 894 WP_416042548.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACK2OW_RS03890 (ACK2OW_03890) yqeB 662668..663390 (+) 723 WP_049141193.1 hypothetical protein -
  ACK2OW_RS03895 (ACK2OW_03895) nucA/comI 663423..663833 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ACK2OW_RS03900 (ACK2OW_03900) sigK 664029..664757 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  ACK2OW_RS03905 (ACK2OW_03905) - 664757..664873 (+) 117 WP_141949575.1 hypothetical protein -
  ACK2OW_RS03910 (ACK2OW_03910) - 664839..665036 (-) 198 Protein_777 recombinase family protein -
  ACK2OW_RS03915 (ACK2OW_03915) - 664994..665172 (-) 179 Protein_778 hypothetical protein -
  ACK2OW_RS03920 (ACK2OW_03920) fumC 665297..666685 (-) 1389 WP_032725151.1 class II fumarate hydratase -
  ACK2OW_RS03925 (ACK2OW_03925) - 666852..667742 (+) 891 WP_077671576.1 LysR family transcriptional regulator -

Sequence


Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=974963 ACK2OW_RS03895 WP_009967785.1 663423..663833(-) (nucA/comI) [Bacillus subtilis strain K3C]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=974963 ACK2OW_RS03895 WP_009967785.1 663423..663833(-) (nucA/comI) [Bacillus subtilis strain K3C]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB P42983

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529


Multiple sequence alignment