Detailed information NTG0097487

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: R8474_RS05715
• Genome accession: NZ_AP026914
• Coordinates: 1127883..1130123 (-)
• Length: 746 a.a.
• NCBI ID: WP_000942403.1
• Uniprot ID: B1IBB4
• Organism: Streptococcus pneumoniae strain PZ900700003
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1122883..1135123

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R8474_RS05680 (PC0003_11300)	-	1123793..1124173 (-)	381	WP_000157154.1	VOC family protein	-
R8474_RS05685 (PC0003_11310)	rplT	1124231..1124590 (-)	360	WP_000124836.1	50S ribosomal protein L20	-
R8474_RS05690 (PC0003_11320)	rpmI	1124642..1124842 (-)	201	WP_001125943.1	50S ribosomal protein L35	-
R8474_RS05695 (PC0003_11330)	infC	1124875..1125405 (-)	531	WP_000848180.1	translation initiation factor IF-3	-
R8474_RS05700 (PC0003_11340)	-	1125712..1126893 (-)	1182	WP_000655958.1	ABC transporter permease	-
R8474_RS05705 (PC0003_11350)	-	1126897..1127484 (-)	588	WP_000933542.1	ATP-binding cassette domain-containing protein	-
R8474_RS05710 (PC0003_11360)	-	1127516..1127704 (-)	189	WP_001809102.1	hypothetical protein	-
R8474_RS05715 (PC0003_11370)	comEC/celB	1127883..1130123 (-)	2241	WP_000942403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
R8474_RS05720 (PC0003_11380)	comEA/celA/cilE	1130107..1130757 (-)	651	WP_000387331.1	ComEA family DNA-binding protein	Machinery gene
R8474_RS05725 (PC0003_11390)	-	1130825..1131394 (-)	570	WP_000443774.1	GNAT family N-acetyltransferase	-
R8474_RS05730	ald	1131571..1132682 (+)	1112	Protein_1133	alanine dehydrogenase	-
R8474_RS05735	-	1132983..1133308 (-)	326	Protein_1134	TfoX/Sxy family protein	-
R8474_RS05740 (PC0003_11440)	-	1133311..1133811 (-)	501	WP_000566982.1	GNAT family N-acetyltransferase	-
R8474_RS05745 (PC0003_11450)	-	1134004..1134972 (-)	969	WP_000658183.1	PhoH family protein	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84530.99 Da
• Isoelectric Point: 9.5181

>NTDB_id=97487 R8474_RS05715 WP_000942403.1 1127883..1130123(-) (comEC/celB) [Streptococcus pneumoniae strain PZ900700003]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCLFIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQN
LADSVERVRILPDTIKVNGDSLSFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLTFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLISLALVYDLRKNIKKLTVLCLLITGLFLLTK
HPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
ENVGDLSEVTKAFHVGEILVSKDSLKQKEFVAELQTTQTKVRSMTVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGLDSWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=97487 R8474_RS05715 WP_000942403.1 1127883..1130123(-) (comEC/celB) [Streptococcus pneumoniae strain PZ900700003]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTATTACTTTGGCTTTATTACGCTATTTT
CTCAGCATCTTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCCGTGGAAATCTGCTG
GTAAAGTTCTAATAATTTGCGGAATCTTTGGATTTTGGTTTGTTTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
CTGGCGGATTCTGTTGAAAGGGTACGGATTTTGCCTGATACTATTAAGGTTAATGGTGATAGTCTATCCTTTCGTGGCAA
GTCTAACGGTCGTGCTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACCGACC
TGCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTAATTACCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAACAATCCAGTCACTTCAAAAGATTGGCAGTTGGGATATAGG
AGAAAACTTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGACGCACTTTCCAGACCCTATGCGCAATTACATGA
CAGGACTCTTGCTGGGACATCTGGACACCGACTTTGAGGAGATGAATGAGCTTTATTCCAGTCTAGGAATTATCCACCTC
TTTGCCCTATCTGGTATGCAGGTAGGTTTTTTCATGAATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTACTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTATTTGACTTGACCTTCTTACCGCTCTTGTCTATT
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATCTTTGAATGGTTGGAGGGCATTATTCGCTTGGT
GTCACAGGTGACAAGTAGACCTCTGGTCTTTGGACAACCCAATGCATGGTTTTTAATCCTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGAAAAAACATTAAAAAGCTAACGGTATTGTGCTTATTGATTACAGGGCTCTTTCTCCTGACCAAG
CATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTACGGGATGTAACTGGGAAAAC
CATTCTCATAGATGTAGGTGGTAAGGCAGAATCTTATAAGAAAATCGAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGAATGTTGGAGATTTGTCAGAGGTGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGACGACTCAAACAAAGGTGCGTAGTATGACAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGACACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAGCTAGCCAACATGGCAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTATCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGTTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (30-176)

• Competence protein (Competence) (216-458)

3D structure

• AlphaFold DB: B1IBB4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus pneumoniae TIGR4	98.123	100	0.981
comEC/celB	Streptococcus pneumoniae D39	97.855	100	0.979
comEC/celB	Streptococcus pneumoniae R6	97.855	100	0.979
comEC/celB	Streptococcus pneumoniae Rx1	97.855	100	0.979
comEC/celB	Streptococcus mitis NCTC 12261	91.946	99.866	0.918
comEC/celB	Streptococcus mitis SK321	91.689	100	0.917
comEC	Lactococcus lactis subsp. cremoris KW2	44.13	99.33	0.438