Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QDT75_RS15695 Genome accession   NZ_CP151078
Coordinates   3316886..3317686 (-) Length   266 a.a.
NCBI ID   WP_179994561.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain PUMA0052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3311886..3322686
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QDT75_RS15675 (QDT75_15675) - 3312951..3313649 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  QDT75_RS15680 (QDT75_15680) hisS 3313678..3314970 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  QDT75_RS15685 (QDT75_15685) ispG 3314967..3316082 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QDT75_RS15690 (QDT75_15690) - 3316104..3316895 (-) 792 WP_272304670.1 helix-turn-helix domain-containing protein -
  QDT75_RS15695 (QDT75_15695) pilF 3316886..3317686 (-) 801 WP_179994561.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QDT75_RS15700 (QDT75_15700) rlmN 3317702..3318934 (-) 1233 WP_272304671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  QDT75_RS15705 (QDT75_15705) ndk 3319057..3319488 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  QDT75_RS15710 (QDT75_15710) iscX 3319602..3319802 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  QDT75_RS15715 (QDT75_15715) - 3319902..3320453 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  QDT75_RS15720 (QDT75_15720) - 3320478..3321776 (-) 1299 WP_001991191.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30075.39 Da        Isoelectric Point: 9.0211

>NTDB_id=974737 QDT75_RS15695 WP_179994561.1 3316886..3317686(-) (pilF) [Acinetobacter baumannii strain PUMA0052]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=974737 QDT75_RS15695 WP_179994561.1 3316886..3317686(-) (pilF) [Acinetobacter baumannii strain PUMA0052]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACATAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729

Multiple sequence alignment