Detailed information NTG0973733

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AABM56_RS19600
• Genome accession: NZ_CP150916
• Coordinates: 4229976..4231118 (-)
• Length: 380 a.a.
• NCBI ID: WP_133861457.1
• Uniprot ID: -
• Organism: Pseudomonas aestiva strain DGS32
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4224976..4236118

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AABM56_RS19580	-	4225784..4226188 (-)	405	WP_133861454.1	TM2 domain-containing protein	-
AABM56_RS19585	-	4226623..4227180 (+)	558	WP_133861455.1	C40 family peptidase	-
AABM56_RS19590	-	4227313..4228524 (+)	1212	WP_017640984.1	putative DNA modification/repair radical SAM protein	-
AABM56_RS19595	-	4228525..4229979 (+)	1455	WP_263259290.1	UdgX family uracil-DNA binding protein	-
AABM56_RS19600	pilU	4229976..4231118 (-)	1143	WP_133861457.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AABM56_RS19605	pilT	4231118..4232154 (-)	1037	Protein_3846	type IV pilus twitching motility protein PilT	-
AABM56_RS19610	-	4232281..4232976 (+)	696	WP_133861458.1	YggS family pyridoxal phosphate-dependent enzyme	-
AABM56_RS19615	proC	4232986..4233804 (+)	819	WP_133861459.1	pyrroline-5-carboxylate reductase	-
AABM56_RS19620	-	4233814..4234407 (+)	594	WP_017640989.1	YggT family protein	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 42400.70 Da
• Isoelectric Point: 7.3062

>NTDB_id=973733 AABM56_RS19600 WP_133861457.1 4229976..4231118(-) (pilU) [Pseudomonas aestiva strain DGS32]
MDFDRFLQLMVDKGASDLFITTGVPPSMKINGRMTPVTKDSLTPDQCRAIVLGVMNDEQRQEFQQKRECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIEVNIPTVEQLRLPEIIKKLAMTKRGLVIFVGATGTGKSTSLAAMIGHRNKNSAGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEIRSRDTMEHALAFAETGHLCLATLHANNANQALDRI
IHFFPADRHGQTWMDLSLNLKGIVAQQLVPTTDGQGRRAVIEVLLNTPLVADLIRKGEVHELKALMKKSGELGMQTFDQS
LYSLYRDGEISYEAALAHADSANDLRLMIKLGSETDADHLNQTTRNLSVDMGDDPPKRFR

Nucleotide

• Length: 1143 bp

>NTDB_id=973733 AABM56_RS19600 WP_133861457.1 4229976..4231118(-) (pilU) [Pseudomonas aestiva strain DGS32]
ATGGATTTCGACAGGTTCCTGCAGCTGATGGTGGACAAGGGGGCGTCCGACCTCTTCATCACCACCGGCGTGCCCCCTTC
GATGAAGATCAACGGGCGCATGACGCCGGTCACCAAGGATTCGCTCACGCCGGATCAGTGCCGAGCCATCGTGCTGGGGG
TGATGAACGACGAGCAGCGCCAGGAATTCCAGCAGAAGCGCGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTTCGGGTCAGCGCCTTCTATCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGGTCAACATCCCCACGGTCGA
GCAGCTGCGCCTGCCGGAGATCATCAAGAAGCTGGCCATGACCAAGCGTGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCCATGATCGGCCACCGCAACAAGAACTCGGCGGGGCACATCATCTCCATCGAGGAC
CCCATCGAATACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCACCGGACGTCATCATGATCGGCGAGATCCGCAGTCGCGACACCATGGAGCACG
CCCTGGCCTTCGCCGAGACCGGTCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCCACTTCTTCCCGGCCGATCGGCACGGCCAGACCTGGATGGACCTGTCGCTCAACCTCAAGGGCATCGTCGCTCAGCA
GCTGGTGCCCACCACCGACGGCCAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTCAACACCCCCTTGGTCGCCGACCTGA
TCCGCAAGGGCGAGGTGCATGAACTCAAGGCGCTGATGAAGAAGTCCGGCGAACTGGGCATGCAGACCTTCGACCAGTCG
CTCTACAGCCTCTATCGTGACGGTGAGATCAGCTACGAAGCGGCCCTGGCCCACGCCGATTCCGCCAATGACCTGCGGCT
GATGATCAAGCTGGGGTCGGAGACGGATGCCGATCACCTCAATCAGACCACCCGCAATCTCTCGGTGGACATGGGCGACG
ATCCGCCCAAGCGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	77.165	100	0.774
pilU	Acinetobacter baylyi ADP1	65.546	93.947	0.616
pilU	Vibrio cholerae strain A1552	56.873	97.632	0.555
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.888	89.211	0.374