Detailed information    

experimental Experimentally validated

Overview

Name   tonB2   Type   Machinery gene
Locus tag   ABD1_RS15030 Genome accession   NC_020547
Coordinates   3190341..3191225 (-) Length   294 a.a.
NCBI ID   WP_000914774.1    Uniprot ID   N9LGJ0
Organism   Acinetobacter baumannii D1279779     
Function   assembly of type IV pilus   
DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.


Genomic Context

Location: 3185341..3196225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABD1_RS15015 (ABD1_29130) miaB 3187188..3188639 (+) 1452 WP_000218141.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  ABD1_RS15020 (ABD1_29140) - 3188677..3189765 (+) 1089 WP_000122983.1 PhoH family protein -
  ABD1_RS15025 (ABD1_29150) ybeY 3189775..3190251 (+) 477 WP_000703571.1 rRNA maturation RNase YbeY -
  ABD1_RS15030 (ABD1_29160) tonB2 3190341..3191225 (-) 885 WP_000914774.1 energy transducer TonB Machinery gene
  ABD1_RS15035 (ABD1_29170) - 3191456..3192127 (+) 672 WP_001193955.1 hypothetical protein -
  ABD1_RS15040 (ABD1_29180) - 3192285..3192998 (+) 714 WP_000201542.1 DUF3108 domain-containing protein -
  ABD1_RS15045 (ABD1_29190) - 3193071..3193646 (+) 576 WP_000543071.1 xanthine phosphoribosyltransferase -
  ABD1_RS15050 (ABD1_29200) wrbA 3193699..3194292 (-) 594 WP_001183348.1 NAD(P)H:quinone oxidoreductase -
  ABD1_RS15055 (ABD1_29210) - 3194579..3195826 (+) 1248 WP_000893466.1 YihY family inner membrane protein -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 33622.48 Da        Isoelectric Point: 9.0935

>NTDB_id=1078 ABD1_RS15030 WP_000914774.1 3190341..3191225(-) (tonB2) [Acinetobacter baumannii D1279779]
MLKKRSLSIEPFYYWWQDRVFIAAIILAILLHIFVLLIHFAMPAPSEQSTKEIAISIRPTEDVIKHADFLAQADQQGSGA
FREAHRMSSNSPAPMPSDASTGEAQLETLEKVQQQRELKFEEKVLMTVLSWQKQAEESQRKKALEQLQSQFQAKAAMVAS
LEAQYLQRQQDFSRQQKIKTVDGIQAKKDASAAYLDKFREKVELYGNRYYPEEAKQQQLKGEVRLMVILNAQGGIRAIRL
LESSGHPVLDEAAKASVRRGAPFGRFDANMKDISELRIIRTWRFDPAEAEFEVH

Nucleotide


Download         Length: 885 bp        

>NTDB_id=1078 ABD1_RS15030 WP_000914774.1 3190341..3191225(-) (tonB2) [Acinetobacter baumannii D1279779]
ATGTTGAAAAAAAGAAGTCTATCCATAGAGCCTTTTTATTATTGGTGGCAAGACCGAGTCTTTATCGCTGCAATAATATT
AGCAATCTTACTGCATATTTTTGTGTTGCTCATCCATTTTGCGATGCCTGCGCCGTCAGAACAATCGACTAAAGAAATTG
CAATATCGATTCGTCCTACTGAAGATGTAATCAAGCATGCAGACTTTTTAGCCCAAGCTGATCAACAAGGTTCAGGGGCT
TTTCGGGAAGCTCATCGGATGTCGAGTAACTCTCCAGCGCCTATGCCAAGTGATGCTTCAACAGGAGAAGCTCAGCTAGA
AACTTTAGAAAAAGTGCAGCAACAAAGAGAACTTAAATTTGAAGAAAAAGTACTCATGACTGTATTGAGTTGGCAGAAAC
AAGCAGAGGAGAGCCAAAGAAAAAAAGCACTTGAACAATTACAAAGTCAGTTTCAGGCAAAAGCTGCCATGGTAGCGAGT
CTTGAAGCTCAGTATTTACAACGTCAGCAAGATTTTAGTCGCCAACAAAAAATAAAAACAGTAGATGGTATTCAAGCCAA
GAAAGATGCGTCGGCAGCTTATCTAGATAAATTTCGTGAAAAAGTAGAGCTTTATGGCAACCGTTATTATCCAGAAGAAG
CGAAACAGCAACAGTTAAAAGGTGAAGTTCGCCTTATGGTAATTTTGAATGCTCAAGGGGGAATTCGTGCAATTCGTTTG
CTTGAAAGCTCAGGTCATCCAGTTCTAGATGAAGCAGCAAAGGCATCGGTACGGCGTGGAGCGCCGTTTGGCCGTTTTGA
TGCAAATATGAAAGATATTTCAGAGCTTCGTATTATTCGTACATGGCGTTTTGATCCAGCTGAAGCTGAATTTGAAGTTC
ATTAA

Domains

Predicted by InterproScan.

Gram-negative bacterial TonB protein C-terminal (TonB_C)

(207-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LGJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB2. Frontiers in Microbiology 12:738034. [PMID: 35126321]