Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   OO861_RS21195 Genome accession   NZ_AP026886
Coordinates   4338635..4340068 (+) Length   477 a.a.
NCBI ID   WP_063143582.1    Uniprot ID   -
Organism   Enterobacter asburiae strain KAM576     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4333635..4345068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO861_RS21165 (KAM576c_41440) - 4334202..4335179 (+) 978 WP_182382750.1 calcium/sodium antiporter -
  OO861_RS21170 (KAM576c_41450) kdsD 4335193..4336179 (+) 987 WP_182382751.1 arabinose-5-phosphate isomerase KdsD -
  OO861_RS21175 (KAM576c_41460) kdsC 4336194..4336760 (+) 567 WP_048981180.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  OO861_RS21180 (KAM576c_41470) lptC 4336757..4337332 (+) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  OO861_RS21185 (KAM576c_41480) lptA 4337301..4337855 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  OO861_RS21190 (KAM576c_41490) lptB 4337862..4338587 (+) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  OO861_RS21195 (KAM576c_41500) rpoN/rpoN1 4338635..4340068 (+) 1434 WP_063143582.1 RNA polymerase factor sigma-54 Machinery gene
  OO861_RS21200 (KAM576c_41510) hpf 4340091..4340378 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  OO861_RS21205 (KAM576c_41520) ptsN 4340461..4340952 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  OO861_RS21210 (KAM576c_41530) rapZ 4340998..4341852 (+) 855 WP_014885397.1 RNase adapter RapZ -
  OO861_RS21215 (KAM576c_41540) npr 4341849..4342121 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  OO861_RS21220 (KAM576c_41550) mtgA 4342161..4342886 (-) 726 WP_039260411.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  OO861_RS21225 (KAM576c_41560) elbB 4342883..4343536 (-) 654 WP_193140158.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54197.95 Da        Isoelectric Point: 4.3173

>NTDB_id=97346 OO861_RS21195 WP_063143582.1 4338635..4340068(+) (rpoN/rpoN1) [Enterobacter asburiae strain KAM576]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=97346 OO861_RS21195 WP_063143582.1 4338635..4340068(+) (rpoN/rpoN1) [Enterobacter asburiae strain KAM576]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGAAGAGATTGAACTTGAGGAGATCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTATCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATTATTAGCGATCATCTGGATCTGCTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCACTCGATCCCCGCCCGGGACAGTCGATCCAAACCAG
CGAGCCCGAGTACGTGATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAAATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482

Multiple sequence alignment