Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WJM97_RS07570 Genome accession   NZ_CP150886
Coordinates   1737984..1739099 (-) Length   371 a.a.
NCBI ID   WP_353932428.1    Uniprot ID   -
Organism   Okeanomitos corallinicola TIOX110     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1732984..1744099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM97_RS07545 (WJM97_07525) - 1734111..1734407 (-) 297 WP_353932423.1 type II toxin-antitoxin system RelE/ParE family toxin -
  WJM97_RS07550 (WJM97_07530) - 1734404..1734634 (-) 231 WP_353932424.1 addiction module protein -
  WJM97_RS07555 (WJM97_07535) - 1734804..1735799 (+) 996 WP_353932425.1 saccharopine dehydrogenase-like oxidoreductase -
  WJM97_RS07560 (WJM97_07540) - 1735869..1736417 (-) 549 WP_353932426.1 hypothetical protein -
  WJM97_RS07565 (WJM97_07545) - 1736622..1737842 (-) 1221 WP_353932427.1 type II secretion system F family protein -
  WJM97_RS07570 (WJM97_07550) pilT 1737984..1739099 (-) 1116 WP_353932428.1 type IV pilus twitching motility protein PilT Machinery gene
  WJM97_RS07575 (WJM97_07555) pilB/pilB1 1739194..1741206 (-) 2013 WP_353932429.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  WJM97_RS07580 (WJM97_07560) grpE 1741646..1742392 (+) 747 WP_353932430.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40589.97 Da        Isoelectric Point: 6.9677

>NTDB_id=973456 WJM97_RS07570 WP_353932428.1 1737984..1739099(-) (pilT) [Okeanomitos corallinicola TIOX110]
MEMMIEDLMEQLIDMGGSDMHLQAGLPPYFRISGKLTPIGDEILTADQCQRLIFSMLNNTQRKTLEQTWELDCAYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDIVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPVEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RVIDVFPADRQTQVRVQLSNSLVAVFSQTLVAKKDPKPGEFGRMMAQEIMIVTPAISNLIREGKTAQIYSAIQTGGKLGM
QTLEKVLADLYKAGTISFEAAMSKTSKPDEVQRLIGATAPPAGAKPGARAR

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=973456 WJM97_RS07570 WP_353932428.1 1737984..1739099(-) (pilT) [Okeanomitos corallinicola TIOX110]
ATGGAAATGATGATTGAAGACTTGATGGAGCAGTTAATTGATATGGGGGGTTCGGATATGCACCTGCAAGCAGGTTTACC
CCCCTACTTCCGTATCAGTGGTAAGCTCACTCCCATCGGTGATGAGATATTAACAGCAGATCAGTGTCAAAGACTGATCT
TTAGTATGCTCAATAATACCCAGCGCAAAACCTTAGAACAAACCTGGGAATTAGATTGTGCTTATGGTGTGAAAGGATTA
GCTCGCTTTCGGGTGAATGTATATAAAGAACGTGGTGCGTATGCTGCTTGTTTACGAGCATTAAGTTCTAAAATTCCTAA
CTTTGAAAAATTAGGTCTACCAGATATTGTTCGAGAAATGTCTGAAAAACCCAGAGGATTAATTTTAGTAACAGGCCCCA
CAGGTTCAGGGAAAACGACAACCCTAGCGGCTATGATTGATTTAATTAACCGGACTAGAGCCGAACATATTTTAACTGTT
GAAGACCCTGTTGAGTTTGTTTATGAACCAATCAAAAGTTTAGTTCACCAACGTCAATTAGGTGAAGATACTAAAAGTTT
TGCGAATGCTTTGAAAGCTGCTTTACGGGAAGATCCAGATATTATTCTGGTAGGGGAAATGCGGGACTTGGAAACTATTT
CTTTGGCAATTTCCGCCGCAGAAACAGGTCACTTAGTTTTTGGTACACTACACACAAGTTCTGCTGCCCAAACCGTTGAC
CGGGTAATTGACGTTTTCCCCGCAGACAGACAAACACAGGTACGTGTACAGTTATCTAACTCTTTAGTTGCAGTTTTCAG
CCAAACCTTAGTAGCCAAAAAAGATCCCAAACCAGGTGAATTTGGGCGGATGATGGCTCAGGAAATTATGATTGTTACCC
CGGCTATTTCTAACCTGATTCGGGAAGGGAAAACAGCCCAAATTTATTCTGCTATTCAAACAGGAGGTAAATTGGGTATG
CAAACTCTAGAAAAAGTTTTAGCTGATTTATACAAAGCTGGCACTATTTCTTTTGAAGCGGCGATGTCTAAGACTTCTAA
ACCTGATGAAGTCCAACGTTTAATTGGTGCTACAGCACCACCAGCAGGTGCAAAACCTGGTGCAAGAGCGCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.467

93.531

0.509
  pilT Pseudomonas stutzeri DSM 10701

53.602

93.531

0.501
  pilT Acinetobacter baylyi ADP1

52.45

93.531

0.491
  pilT Acinetobacter baumannii strain A118

52.161

93.531

0.488
  pilT Legionella pneumophila strain Lp02

52.161

93.531

0.488
  pilT Legionella pneumophila strain ERS1305867

52.161

93.531

0.488
  pilT Acinetobacter nosocomialis M2

52.161

93.531

0.488
  pilT Acinetobacter baumannii D1279779

52.161

93.531

0.488
  pilT Neisseria gonorrhoeae MS11

50.147

91.375

0.458
  pilT Neisseria meningitidis 8013

49.853

91.375

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.123

92.183

0.453
  pilT Vibrio cholerae strain A1552

49.123

92.183

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48

94.34

0.453
  pilU Acinetobacter baylyi ADP1

38.244

95.148

0.364
  pilU Pseudomonas stutzeri DSM 10701

37.96

95.148

0.361

Multiple sequence alignment