Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   WF295_RS13625 Genome accession   NZ_CP150885
Coordinates   2723468..2724502 (-) Length   344 a.a.
NCBI ID   WP_041817253.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain Baplich1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2718468..2729502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WF295_RS13575 (WF295_13575) - 2718538..2719332 (-) 795 WP_020452167.1 TasA family protein -
  WF295_RS13580 (WF295_13580) - 2719405..2719989 (-) 585 WP_020452168.1 signal peptidase I -
  WF295_RS13585 (WF295_13585) tapA 2719986..2720714 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  WF295_RS13590 (WF295_13590) - 2720992..2721312 (+) 321 WP_020452170.1 YqzG/YhdC family protein -
  WF295_RS13595 (WF295_13595) - 2721342..2721524 (-) 183 WP_020452171.1 YqzE family protein -
  WF295_RS13600 (WF295_13600) comGG 2721613..2721978 (-) 366 WP_020452172.1 competence type IV pilus minor pilin ComGG -
  WF295_RS13605 (WF295_13605) comGF 2721990..2722478 (-) 489 WP_236613657.1 competence type IV pilus minor pilin ComGF -
  WF295_RS13610 (WF295_13610) comGE 2722387..2722734 (-) 348 WP_020452174.1 competence type IV pilus minor pilin ComGE -
  WF295_RS13615 (WF295_13615) comGD 2722718..2723155 (-) 438 WP_020452175.1 competence type IV pilus minor pilin ComGD -
  WF295_RS13620 (WF295_13620) comGC 2723161..2723454 (-) 294 WP_020452176.1 competence type IV pilus major pilin ComGC Machinery gene
  WF295_RS13625 (WF295_13625) comGB 2723468..2724502 (-) 1035 WP_041817253.1 competence type IV pilus assembly protein ComGB Machinery gene
  WF295_RS13630 (WF295_13630) comGA 2724492..2725556 (-) 1065 WP_020452178.1 competence type IV pilus ATPase ComGA Machinery gene
  WF295_RS13635 (WF295_13635) - 2725714..2726553 (-) 840 WP_020452179.1 STAS domain-containing protein -
  WF295_RS13645 (WF295_13645) - 2726795..2727172 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  WF295_RS13650 (WF295_13650) - 2727383..2727628 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  WF295_RS13655 (WF295_13655) - 2727664..2728302 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -
  WF295_RS13660 (WF295_13660) - 2728458..2728631 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  WF295_RS13665 (WF295_13665) - 2728692..2729006 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39780.12 Da        Isoelectric Point: 9.7341

>NTDB_id=973421 WF295_RS13625 WP_041817253.1 2723468..2724502(-) (comGB) [Bacillus paralicheniformis strain Baplich1]
MKIKKKWPVREQADILKKLGDMMKSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGRPVFQVLEMISFHKDAVTIVYF
AEQHGNLPYAFRQSGELLHRKIAQTEKLKKAARYPLFLVLTVCVIISIMKSAIIPQFSAIYESMNVETSFATTLIFSVFD
HFYLFIAGMMLIAAALSLYYLCSFRHKPPEDKMTFLIRIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LSFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGLLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=973421 WF295_RS13625 WP_041817253.1 2723468..2724502(-) (comGB) [Bacillus paralicheniformis strain Baplich1]
ATGAAGATTAAAAAGAAGTGGCCCGTCCGGGAACAGGCGGACATTCTGAAAAAACTCGGAGACATGATGAAGAGCGGATA
TACGATTTTGGATGCTTTAAGTATGCTGAAACTGCAATTGAATGAAAGGCGAAGAGCAGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGCGACCTGTGTTTCAAGTGCTTGAAATGATTTCATTTCATAAGGATGCTGTTACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCATATGCCTTTCGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAACCGAAAA
ACTAAAGAAAGCCGCCAGATATCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATTATCTCTATTATGAAATCGGCAATCA
TCCCGCAATTTTCCGCGATCTATGAATCAATGAACGTTGAAACGTCTTTTGCAACGACCCTCATCTTTTCGGTTTTTGAT
CATTTTTATCTTTTTATCGCAGGAATGATGTTGATTGCCGCCGCTTTATCCCTCTATTATCTGTGTTCATTTCGCCATAA
ACCTCCAGAAGACAAAATGACCTTTCTCATTAGAATTCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGTTATTTTT
TATCGCTCCAATTGAGCAATCTTTTGCAAGCTGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTATCATTTCATAAAAATGAAGCGAAGCGTCTGATCGAGCGGCTGAAACAAGGGGAGAGTCTGGAGCAGATGCTGGCCGG
GCATCCGTTTTATGAAAATGATCTGGCGAAGGCTGTTGCTCACGGCCAATTAAACGGACTCTTATACAGAGAGCTGTATT
CATACAGTCAATTTTTGATTGAACGGCTTGAACGGAAAGCAGAAAAATGGACAGGTTTGATCCAGCCATTGATTTACGGG
CTAACAGCGGCGATGATATTAATTCTTTATTTGTCTATGCTTTTGCCAATGTATCAAATGATGAACCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

57.276

93.895

0.538

Multiple sequence alignment