Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   WF295_RS06370 Genome accession   NZ_CP150885
Coordinates   1270547..1274233 (+) Length   1228 a.a.
NCBI ID   WP_020450870.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain Baplich1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1265547..1279233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WF295_RS06360 (WF295_06360) modB 1266294..1266950 (+) 657 WP_003180426.1 molybdate ABC transporter permease subunit -
  WF295_RS06365 (WF295_06365) addB 1267060..1270560 (+) 3501 WP_020450869.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  WF295_RS06370 (WF295_06370) addA 1270547..1274233 (+) 3687 WP_020450870.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  WF295_RS06375 (WF295_06375) sbcD 1274299..1275480 (+) 1182 WP_003180431.1 exonuclease subunit SbcD -
  WF295_RS06380 (WF295_06380) sbcC 1275477..1278869 (+) 3393 WP_020450871.1 exonuclease subunit SbcC -
  WF295_RS06385 (WF295_06385) - 1278914..1279132 (-) 219 WP_016885923.1 spore germination protein -

Sequence

Protein


Download         Length: 1228 a.a.        Molecular weight: 141105.14 Da        Isoelectric Point: 5.6938

>NTDB_id=973396 WF295_RS06370 WP_020450870.1 1270547..1274233(+) (addA) [Bacillus paralicheniformis strain Baplich1]
MEISKPKGSNWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQEHPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKQDPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHTFVDLYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPRGPAPRAENFLDDLAQIDRLIQQQNDFSALYELVPAVSFQRLKPCKGDAYDPRLVDEAADLRNSAKKQLEKLK
SDYFMRTPEQHLESLREMKPVIQTLVQLVLEYGKRFADAKKEKAIVDFSDLEHDCLAILSGKNQAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDNGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPSNKATITELLLIENQSGAQDEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLAAADGDERLSEKLRRFDALLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRSQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPLVIDEIREHPARFQVSWLSAADLQAE
DIDQAGEERHGRLSQIQMGLPIDGSFEYEQQVRERLTWSYPYKDAAKVRTKQSVSEMKRQKEYEYGDRSLIRPTKDALLF
KRPSFMMAKGLTAAERGTAMHTVMQHIPLTRALEMNELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLLGAR
RVEREVPFNMTLSAKEVYPDLESADETVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQLY
ARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide


Download         Length: 3687 bp        

>NTDB_id=973396 WF295_RS06370 WP_020450870.1 1270547..1274233(+) (addA) [Bacillus paralicheniformis strain Baplich1]
ATGGAGATTTCTAAACCGAAGGGCAGCAATTGGACTGATGACCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGCTCCGGAAAAACGGCCGTGCTGGTTGAGCGGATCATTCGGAAAATAACGGATCAGGAACATC
CCGTAGATGTCGACAGGCTGCTTGTCGTCACCTTTACGAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCAGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCACTCATTCTGTCTTCAAGTCATCCGCAAATACTATTATTTGATCGATGTTGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTTGGCGATGAGGTGCTGGACGAGCTGTTTGAAGAAGAATACAAAAAGCAAGATCCGGCC
TTTTTTGAACTTGTGGACAGATATACGACAGACCGTCATGACCTCGATTTGCAGCATCTCGTCAAGCGGGTCTACGAGTT
TTCCCGTTCGCATCCCGATCCGGAAGGCTGGCTTCACACATTTGTTGATCTGTATGACGCGGCTTCAGATACGAAAGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAGGGGATGCGGCAAAAGCTGACGCGTGCG
CTTGATTTGACAAAGCAGCCAAGGGGGCCTGCGCCGCGCGCCGAAAATTTCCTCGATGATTTAGCGCAGATTGACAGGCT
GATTCAGCAGCAGAATGATTTTTCGGCTCTGTACGAGCTTGTACCGGCCGTTTCGTTTCAAAGATTGAAGCCGTGCAAAG
GAGATGCGTACGATCCTCGATTGGTTGATGAGGCCGCAGATCTCCGCAACAGCGCGAAAAAGCAACTTGAAAAGCTGAAA
AGCGATTATTTTATGCGAACTCCCGAGCAGCACCTTGAGAGCTTGAGGGAAATGAAGCCGGTCATTCAGACGCTCGTCCA
GCTCGTGTTGGAGTATGGCAAGCGGTTTGCGGACGCGAAAAAGGAAAAAGCGATCGTCGATTTCTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGGGAAAAACCAAGCCGGCGAAGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCATGAAGTGCTTGTTGATGAATATCAGGATACGAACTTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCAGAACCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGACAATGGCTCGGGTTCCGGTCAAAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAGCAGCTCATGGGAGAAAAGGTCGGGGAAGTCGAATATGACGAACA
GGCCGAATTGAAGCTTGGCGCGTCTTATCCGTCGAACAAAGCGACAATAACGGAGCTTCTGCTCATCGAAAACCAGAGCG
GCGCGCAGGATGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGACAAATCCGCAGGCTG
ATAACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCACGGAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCGCAAATTATGGACGAGTTTAAACAGCAGGGGATCCCGGTATACGCGAACCTCTCAACGG
GTTATTTTGAAGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCTTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTAGGTCTTGATGAAAATGAGCTTTCCTTGATCAGAATCAAGGATAAAAAAGCGCC
GTTTTACGAAGCGATGAAAGCATACCTTGCTGCAGCAGATGGCGATGAAAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
CCCTGCTCAAAAAGTGGAGAGCGTATGCGAAAAACCACTCTGTGGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGACAGGCCAATTTGCGCGCGCTTTACGACAGGGCAAGATC
CTATGAAGCGACATCGTTCCGCGGTTTGTTCCGCTTTTTAAGGTTTATTGAGCGGATGCAGGAGCGGGGTGATGATCTCG
GAACGGCAAGAGCTCTGAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAGTCTTATCTGCTGGATAAAGAACTCGG
TTTCGGAACGAAGTTTATTCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTGATCGCGATGAAGAAAAAGCTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCTCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCTAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGTCACAAGCGGCGGGGTCTGAGTGGCTGCTTCCGGATTATGA
ACGGTTTCAGGCGAAATCTTACCTGGATTTCATCGGACCTGCTTTGATGAGACACAGAGATATGGATGAATCCGGCGCGC
CGCTGGTGATTGATGAGATCCGTGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCGGCCGCGGATCTTCAAGCAGAA
GATATTGATCAGGCCGGTGAAGAAAGACACGGCCGGCTCTCCCAAATTCAAATGGGGCTTCCGATCGATGGTTCGTTTGA
GTATGAGCAACAAGTCAGAGAAAGGCTGACATGGTCATATCCATACAAAGACGCCGCCAAAGTCCGAACAAAACAGTCTG
TCTCAGAAATGAAAAGGCAGAAGGAGTATGAGTACGGCGACCGCTCGCTGATTCGCCCAACTAAGGATGCTCTCCTGTTT
AAACGGCCGTCCTTTATGATGGCAAAAGGCCTGACCGCGGCCGAACGAGGGACGGCGATGCACACCGTCATGCAGCATAT
TCCGCTGACCCGTGCGCTTGAAATGAATGAGCTCAGCCGCCTCCTAGACAGGCTTGTTGAGAAAGAGCTATTAACAGAAG
ACCAGCGGGCAGCGATTGAGGAAGACGATATTCTCGCATTCTTTGATACGGAAATCGGACAAAAGCTTCTCGGCGCCCGC
CGCGTCGAACGGGAAGTTCCCTTTAACATGACGCTGTCCGCCAAAGAGGTTTATCCTGATCTTGAGTCAGCCGACGAAAC
TGTATTGATACAGGGAATTATCGATTGTTTATTCGAGACGGAGGACGGTTTTTACCTTCTTGATTACAAAACCGACCGGA
TACACGGAAAGTACCGAAACGGATTCGAAGGGGCCGAACCGATTTTAAGGAAGCGTTACGAAACACAAATTCAGCTTTAT
GCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGTGCACTGTACTTTTTTGACGGAGGACATGTGCTCTT
ATTTTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(471-880)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-460)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1049-1220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

71.73

100

0.719

Multiple sequence alignment