Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WN985_RS15290 Genome accession   NZ_CP150849
Coordinates   3312587..3314092 (+) Length   501 a.a.
NCBI ID   WP_342307790.1    Uniprot ID   -
Organism   Burkholderia pyrrocinia strain BX1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3307587..3319092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WN985_RS15265 (WN985_15265) kdsD 3308830..3309813 (+) 984 WP_096471802.1 arabinose 5-phosphate isomerase KdsD -
  WN985_RS15270 (WN985_15270) - 3309813..3310349 (+) 537 WP_047899045.1 HAD family hydrolase -
  WN985_RS15275 (WN985_15275) lptC 3310353..3310967 (+) 615 WP_342307785.1 LPS export ABC transporter periplasmic protein LptC -
  WN985_RS15280 (WN985_15280) lptA 3310988..3311653 (+) 666 WP_342307787.1 lipopolysaccharide transport periplasmic protein LptA -
  WN985_RS15285 (WN985_15285) lptB 3311650..3312426 (+) 777 WP_096471805.1 LPS export ABC transporter ATP-binding protein -
  WN985_RS15290 (WN985_15290) rpoN/rpoN1 3312587..3314092 (+) 1506 WP_342307790.1 RNA polymerase factor sigma-54 Machinery gene
  WN985_RS15295 (WN985_15295) raiA 3314250..3314609 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  WN985_RS15300 (WN985_15300) - 3314779..3315318 (+) 540 WP_059526091.1 PTS sugar transporter subunit IIA -
  WN985_RS15305 (WN985_15305) hprK 3315448..3316416 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  WN985_RS15310 (WN985_15310) rapZ 3316492..3317400 (+) 909 WP_096471807.1 RNase adapter RapZ -
  WN985_RS15315 (WN985_15315) - 3317428..3318063 (+) 636 WP_065499174.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54709.79 Da        Isoelectric Point: 4.8237

>NTDB_id=973039 WN985_RS15290 WP_342307790.1 3312587..3314092(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain BX1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPGQNTEPAP
ANSGGTSGDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPDELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=973039 WN985_RS15290 WP_342307790.1 3312587..3314092(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain BX1]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACACCCCAGCTACAGCAGTCGATCCGGCTTCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCCGCGGACGGATCGCTGATCGCGCAGACACCTCCCGGCCAGAACACCGAGCCGGCCCCG
GCAAACAGCGGCGGCACGTCGGGCGATCGCGCGGAGCGCGACGAGCCGGCCGGTGCCGACGAATACGACGGTTATGGGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGATGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCTGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGGACGAACTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTTCAGGAAGCACGCTGGCTGATCAAGAACATTCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACTGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCAACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691

Multiple sequence alignment