Detailed information NTG0972852

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: WN777_RS25750
• Genome accession: NZ_CP150839
• Coordinates: 4960117..4961466 (-)
• Length: 449 a.a.
• NCBI ID: WP_242294454.1
• Uniprot ID: -
• Organism: Bacillus pacificus strain SG15
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4955117..4966466

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WN777_RS25730 (WN777_25710)	secA	4955439..4957946 (-)	2508	WP_048553050.1	preprotein translocase subunit SecA	-
WN777_RS25735 (WN777_25715)	hpf	4958223..4958765 (-)	543	WP_087955105.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
WN777_RS25740 (WN777_25720)	cspC	4959089..4959286 (-)	198	WP_001162041.1	cold shock protein CspC	-
WN777_RS25745 (WN777_25725)	-	4959413..4960117 (-)	705	WP_242294455.1	ComF family protein	-
WN777_RS25750 (WN777_25730)	comFA	4960117..4961466 (-)	1350	WP_242294454.1	ATP-dependent helicase ComFA	Machinery gene
WN777_RS25755 (WN777_25735)	-	4961593..4963008 (-)	1416	WP_113712272.1	NlpC/P60 family protein	-
WN777_RS25760 (WN777_25740)	-	4963155..4963469 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
WN777_RS25765 (WN777_25745)	-	4963643..4964485 (-)	843	WP_000844764.1	DegV family protein	-
WN777_RS25770 (WN777_25750)	-	4964725..4965360 (+)	636	WP_139868377.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 50937.49 Da
• Isoelectric Point: 9.5961

>NTDB_id=972852 WN777_RS25750 WP_242294454.1 4960117..4961466(-) (comFA) [Bacillus pacificus strain SG15]
MLAGKQLLLEELSSDLQREISNLKEKGEIVCVQGVIKKASKYICQRCGNIKQRLFASFLCKRCGKACAYCRKCITMGRVS
ECTVLVRGIHERNGDRELNPLQWNGALSIGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELAPRLQEVFPSITVAALYGGSVDHKKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKHNFIRGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLQHKKIPRVLLQWLKMNLNKKYPIFL
FVPHVRYIEEISQLLKGLDNRIDGVHAEDSMRKEKVASFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVMYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=972852 WN777_RS25750 WP_242294454.1 4960117..4961466(-) (comFA) [Bacillus pacificus strain SG15]
ATGCTTGCTGGAAAACAGTTGCTATTAGAAGAACTCTCTTCAGATTTACAGAGAGAAATAAGTAATTTAAAAGAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAAAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCGGTAAAGCATGCGCGTATTGCCGGAAGTGTATCACGATGGGGAGAGTTAGC
GAATGTACTGTACTTGTTCGCGGGATTCATGAAAGAAACGGAGACAGGGAGCTAAATCCATTACAGTGGAATGGGGCTTT
ATCGATTGGTCAGGAGTTGGCGGCGCAAGGAGTTATAGAAGCGATTAAGCAGAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCGCTCCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGCGCCGAGATTACAAGAAGTGTTTCCAAGTATAACTGTAGCTGCTTTATACGG
AGGGAGTGTAGATCATAAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAACTGTTACGTTACTATAGAGCGTTTC
ATGTCATGATTGTAGATGAGATTGATGCCTTCCCATATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCACAATTTCATCAGGGGGAAACAAAA
AGGTATCATTGTCTCAGGGCGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCAGCATAAAAAAATTCCTCGCGTGTTACTACAATGGTTAAAAATGAACTTAAACAAAAAGTATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAAGTCAGTTGTTGAAAGGGTTGGATAATAGAATCGATGGCGTACATGC
AGAAGACTCGATGAGAAAAGAAAAAGTAGCATCTTTCAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGAGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGAGCCGAAGAAGAAATTTTTTCAGAGAGTGCGCTCGTA
CAAATTGCAGGGCGAGCGGGGCGTAGTTTTGAAGAGCCGTATGGTGAGGTTATGTATTTTCATTACGGTAAGACAGAGGC
AATGGTACGTGCGAAAAAACATATTCAAAGTATGAACAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (110-254)

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.126	98.886	0.506
comFA	Latilactobacillus sakei subsp. sakei 23K	40.394	90.423	0.365