Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WN777_RS21100 Genome accession   NZ_CP150839
Coordinates   4098985..4100028 (-) Length   347 a.a.
NCBI ID   WP_073530042.1    Uniprot ID   -
Organism   Bacillus pacificus strain SG15     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4093985..4105028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WN777_RS21055 (WN777_21040) - 4094414..4094614 (-) 201 WP_000106079.1 YqzE family protein -
  WN777_RS21060 (WN777_21045) aroK 4094653..4095150 (-) 498 WP_139866678.1 shikimate kinase AroK -
  WN777_RS21065 (WN777_21050) - 4095269..4095919 (-) 651 WP_242294015.1 2OG-Fe(II) oxygenase -
  WN777_RS21070 (WN777_21055) comGG 4096095..4096466 (-) 372 WP_242294016.1 competence type IV pilus minor pilin ComGG -
  WN777_RS21075 (WN777_21060) comGF 4096463..4096933 (-) 471 WP_000923056.1 competence type IV pilus minor pilin ComGF -
  WN777_RS21080 (WN777_21065) comGE 4096903..4097205 (-) 303 WP_000829518.1 competence type IV pilus minor pilin ComGE -
  WN777_RS21085 (WN777_21070) comGD 4097198..4097653 (-) 456 WP_139866672.1 comG operon protein ComGD -
  WN777_RS21090 (WN777_21075) comGC 4097650..4097949 (-) 300 WP_001178694.1 competence type IV pilus major pilin ComGC -
  WN777_RS21095 (WN777_21080) comGB 4097961..4098992 (-) 1032 WP_139866670.1 competence type IV pilus assembly protein ComGB -
  WN777_RS21100 (WN777_21085) comGA 4098985..4100028 (-) 1044 WP_073530042.1 competence protein ComGA Machinery gene
  WN777_RS21105 (WN777_21090) - 4100234..4100929 (+) 696 WP_029439920.1 metalloregulator ArsR/SmtB family transcription factor -
  WN777_RS21110 (WN777_21095) - 4101055..4101297 (+) 243 WP_000440714.1 DUF2626 domain-containing protein -
  WN777_RS21115 (WN777_21100) - 4101405..4102799 (+) 1395 WP_001094339.1 L-cystine transporter -
  WN777_RS21120 (WN777_21105) - 4102974..4103381 (-) 408 WP_062821281.1 hypothetical protein -
  WN777_RS21125 (WN777_21110) - 4103469..4103684 (-) 216 WP_001008330.1 DUF3912 family protein -
  WN777_RS21130 (WN777_21115) - 4103938..4104249 (+) 312 WP_001093243.1 hypothetical protein -
  WN777_RS21135 (WN777_21120) - 4104285..4104773 (-) 489 WP_242294416.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39343.81 Da        Isoelectric Point: 9.1826

>NTDB_id=972841 WN777_RS21100 WP_073530042.1 4098985..4100028(-) (comGA) [Bacillus pacificus strain SG15]
MNGIESFANTILKEACRVQASDLHIVPRKKDVVIQLRIGKDLMTKQYIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
IQIDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTVCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESSLRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=972841 WN777_RS21100 WP_073530042.1 4098985..4100028(-) (comGA) [Bacillus pacificus strain SG15]
ATGAATGGAATTGAAAGCTTTGCGAATACGATTTTGAAAGAGGCGTGTAGGGTACAAGCTTCGGATTTACATATTGTGCC
CCGGAAGAAGGATGTAGTGATTCAACTGCGCATAGGGAAAGATTTAATGACTAAACAATACATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGAGAGAGGCGGAAACCACAAAACGGTTCACTATAT
ATACAAATAGATGGACAAGAAGTGTATTTACGCCTTTCCACGCTTCCAACCGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCGTCTATTCAGCCGCTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
ATTATTCCCATGGATTACTCGTATTTACGGGACCGACTGGTTCAGGAAAGACAACAACAATGTATGCATTACTAGAGGTG
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTGG
GTGAAATTCGTGATGAAGAAACAGCAAAAATCGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACACTGCAT
ACGAATGATGCGAAAGGAGCAATACTTCGGTTCATGGATTTTGGTATAACGAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTTGAATTGAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTGTATGTAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACGTACAAGCATGAGACATTAGAGTCTTCGCTGCGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.213

100

0.582
  pilB Vibrio campbellii strain DS40M4

35.694

100

0.363

Multiple sequence alignment