Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   WN777_RS19880 Genome accession   NZ_CP150839
Coordinates   3893793..3894227 (-) Length   144 a.a.
NCBI ID   WP_000811499.1    Uniprot ID   A0A1J9ZQC5
Organism   Bacillus pacificus strain SG15     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3888793..3899227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WN777_RS19865 (WN777_19850) - 3889816..3890373 (+) 558 WP_139866858.1 PadR family transcriptional regulator -
  WN777_RS19870 (WN777_19855) metE 3891042..3893330 (+) 2289 WP_139866856.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  WN777_RS19875 (WN777_19860) comJ 3893368..3893757 (-) 390 WP_000424015.1 competence protein ComJ -
  WN777_RS19880 (WN777_19865) nucA/comI 3893793..3894227 (-) 435 WP_000811499.1 DNA-entry nuclease Machinery gene
  WN777_RS19885 (WN777_19870) - 3894617..3895366 (+) 750 WP_000388461.1 DUF3967 domain-containing protein -
  WN777_RS19890 (WN777_19875) kinB 3895420..3896694 (-) 1275 WP_139866854.1 sporulation sensor histidine kinase KinB -
  WN777_RS19895 (WN777_19880) - 3897004..3897204 (+) 201 WP_000929285.1 hypothetical protein -
  WN777_RS19900 (WN777_19885) - 3897328..3898491 (+) 1164 WP_062821082.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15996.35 Da        Isoelectric Point: 8.4331

>NTDB_id=972838 WN777_RS19880 WP_000811499.1 3893793..3894227(-) (nucA/comI) [Bacillus pacificus strain SG15]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKDRYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=972838 WN777_RS19880 WP_000811499.1 3893793..3894227(-) (nucA/comI) [Bacillus pacificus strain SG15]
ATGAAACAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTGCTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCATATGATCAAGTTCTAGAATTTCCGAAAGATCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCCGATAACCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J9ZQC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

72.072

77.083

0.556

Multiple sequence alignment