Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   WN777_RS05780 Genome accession   NZ_CP150839
Coordinates   1130295..1134020 (+) Length   1241 a.a.
NCBI ID   WP_318160855.1    Uniprot ID   -
Organism   Bacillus pacificus strain SG15     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1125295..1139020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WN777_RS05765 (WN777_05755) - 1125453..1126046 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  WN777_RS05770 (WN777_05760) lepB 1126103..1126666 (+) 564 WP_048526650.1 signal peptidase I -
  WN777_RS05775 (WN777_05765) addB 1126783..1130298 (+) 3516 WP_242294487.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  WN777_RS05780 (WN777_05770) addA 1130295..1134020 (+) 3726 WP_318160855.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  WN777_RS05785 (WN777_05775) - 1134033..1134320 (+) 288 WP_139868728.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  WN777_RS05790 (WN777_05780) gerPF 1134425..1134640 (-) 216 WP_001141566.1 spore germination protein GerPF -
  WN777_RS05795 (WN777_05785) - 1134683..1135069 (-) 387 WP_139868726.1 spore germination protein GerPE -
  WN777_RS05800 (WN777_05790) gerPD 1135085..1135279 (-) 195 WP_001102341.1 spore germination protein GerPD -
  WN777_RS05805 (WN777_05795) gerPC 1135286..1135900 (-) 615 WP_001070749.1 spore germination protein GerPC -
  WN777_RS05810 (WN777_05800) gerPB 1135969..1136175 (-) 207 WP_001012508.1 spore germination protein GerPB -
  WN777_RS05815 (WN777_05805) gerPA 1136190..1136411 (-) 222 WP_001111187.1 spore germination protein GerPA -
  WN777_RS05820 (WN777_05810) - 1136508..1136687 (-) 180 WP_000462846.1 aspartyl-phosphate phosphatase Spo0E family protein -
  WN777_RS05825 (WN777_05815) - 1136935..1137834 (+) 900 WP_242294485.1 fumarylacetoacetate hydrolase family protein -
  WN777_RS05830 (WN777_05820) - 1137870..1138154 (-) 285 WP_000926868.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142767.07 Da        Isoelectric Point: 4.8834

>NTDB_id=972819 WN777_RS05780 WP_318160855.1 1130295..1134020(+) (addA) [Bacillus pacificus strain SG15]
MMENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
NNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAIKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVLDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEERELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQIAGMVNKELLTFEQAEEIAIEKLISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIAGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=972819 WN777_RS05780 WP_318160855.1 1130295..1134020(+) (addA) [Bacillus pacificus strain SG15]
ATGATGGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGGCG
TGATATTTTAGTCGCAGCAGCAGCAGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGCTCGCAGCACATTCGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCTATTTCGACGATCCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
AATAATACGATATTCTTTGAGCTCGTTGATCGTTATACGAGTGATCGTAGTGACGATGATTTACAGCGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCGCATCCAAATCCTGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTATTTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAGCATATT
CGGAAAGCGACGGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACACTGCAAGCAGATTTAGCTCTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGCGATTACAACGAGGATATTGTCAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCGGTATTAGA
GAAGCTTGTTCAACTTGTAAAAGTATTTACCGAGCGTTTCCAAGCGATAAAACGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAAGCCATCTGCAGTAGCGCTTCAA
TATCGCAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAATTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTAGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGCTTCACGCAAGAAGGATTAGGCGGCGGAATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTATTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
TGACTACGATGCTGACGCTGAATTAAAGTTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGTA
TTCAGCAAACAGAAGAAGAAGTGTTAGACGGTGAAGAAGGTGCGGAAGTAGAAAAAGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCGGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCACCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAAAAAGGCTCGTTTTATGAAGTGATGAGCTCCTTCTTAAAAGGGGCACCGCTTGAAGAAGAACGAGAAC
TACATGATAAATTAGAGTGGTTCTATAATTTACTACAAGGATGGCGTGAATTTGCACGCCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGATTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGACAGGCAAACTT
ACGCGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTAGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTAGCTGGACTAGGTCGCCGCTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGACTTCGGTTTTGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGTGTATTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGAC
ATCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGGAGCATTCCAGATGAAATTTATGGATACGACACGAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTCGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGAAGAAGTGTATGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCATCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAATTACGTGCACCAATTCAAACACGTCCTCGTTTTATGGAGAAAAAGGGATTAACATACGCAGAGCGCGG
AACAGCAGTACATGCTGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTCGAGCAGGCGGAAGAAATTGCAATTGAAAAATTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCGGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGCGGGGAATCCATTCTTGTCCAAGGGGTTATTGACTGCATGATCGAAGAGGAAGATG
GTATTACGTTAATCGACTTCAAAACGGATACGATTGCAGGAAAATTCCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTCGAGAAAAGCTTACAACACCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTGTAAAAATCGAAGAATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.666

100

0.537

Multiple sequence alignment