Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   WNC56_RS09040 Genome accession   NZ_CP150833
Coordinates   1798100..1798480 (+) Length   126 a.a.
NCBI ID   WP_044335941.1    Uniprot ID   A0AAP7UKB4
Organism   Bacillus safensis strain DMB13     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1764917..1802894 1798100..1798480 within 0


Gene organization within MGE regions


Location: 1764917..1802894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WNC56_RS08875 (WNC56_08865) - 1764917..1765756 (+) 840 WP_024424079.1 hypothetical protein -
  WNC56_RS08880 (WNC56_08870) - 1765801..1766182 (+) 382 Protein_1701 ArpU family phage packaging/lysis transcriptional regulator -
  WNC56_RS08885 (WNC56_08875) - 1766312..1767897 (+) 1586 Protein_1702 ribonuclease YeeF family protein -
  WNC56_RS08890 (WNC56_08880) - 1767902..1768285 (+) 384 WP_024425095.1 immunity 22 family protein -
  WNC56_RS08895 (WNC56_08885) - 1768359..1768763 (+) 405 WP_024425096.1 hypothetical protein -
  WNC56_RS08900 (WNC56_08890) - 1769229..1769411 (+) 183 WP_073980085.1 hypothetical protein -
  WNC56_RS08905 (WNC56_08895) - 1769503..1769847 (+) 345 WP_024425098.1 hypothetical protein -
  WNC56_RS08910 (WNC56_08900) - 1770166..1770534 (+) 369 WP_024425099.1 nuclear transport factor 2 family protein -
  WNC56_RS08915 (WNC56_08905) - 1770913..1772004 (+) 1092 WP_044335959.1 Rap family tetratricopeptide repeat protein -
  WNC56_RS08920 (WNC56_08910) - 1772007..1772141 (+) 135 WP_259279673.1 hypothetical protein -
  WNC56_RS08925 (WNC56_08915) - 1772265..1774193 (+) 1929 WP_270125055.1 T7SS effector LXG polymorphic toxin -
  WNC56_RS08930 (WNC56_08920) - 1774197..1774682 (+) 486 WP_103132382.1 immunity protein YezG family protein -
  WNC56_RS08935 (WNC56_08925) - 1774856..1775581 (+) 726 WP_122631424.1 DNA/RNA non-specific endonuclease -
  WNC56_RS08940 (WNC56_08930) - 1775588..1776064 (+) 477 WP_103132505.1 immunity protein YezG family protein -
  WNC56_RS08945 (WNC56_08935) - 1776128..1776589 (-) 462 WP_044335609.1 macro domain-containing protein -
  WNC56_RS08950 (WNC56_08940) - 1776818..1777399 (+) 582 WP_103132504.1 undecaprenyl-diphosphatase -
  WNC56_RS08955 (WNC56_08945) - 1777449..1777820 (-) 372 WP_103132503.1 iron chaperone -
  WNC56_RS08960 (WNC56_08950) - 1778363..1779475 (+) 1113 WP_103132502.1 tetratricopeptide repeat protein -
  WNC56_RS08965 (WNC56_08955) - 1780204..1780899 (-) 696 WP_103132512.1 YoaK family protein -
  WNC56_RS08970 (WNC56_08960) - 1780967..1782799 (-) 1833 WP_103132501.1 DNA ligase D -
  WNC56_RS08975 (WNC56_08965) - 1782915..1783763 (+) 849 WP_024425113.1 Ku protein -
  WNC56_RS08980 (WNC56_08970) - 1783787..1784230 (-) 444 WP_041109183.1 DUF2188 domain-containing protein -
  WNC56_RS08985 (WNC56_08975) - 1784391..1784864 (+) 474 WP_052503034.1 VOC family protein -
  WNC56_RS08990 (WNC56_08980) - 1784913..1785326 (+) 414 WP_103132500.1 Rrf2 family transcriptional regulator -
  WNC56_RS08995 (WNC56_08985) - 1785427..1786278 (+) 852 WP_103132499.1 SDR family oxidoreductase -
  WNC56_RS09000 (WNC56_08990) - 1786433..1787341 (+) 909 WP_103132498.1 DMT family transporter -
  WNC56_RS09005 (WNC56_08995) - 1787354..1787545 (-) 192 WP_044335590.1 TIGR00366 family protein -
  WNC56_RS09010 (WNC56_09000) - 1787656..1789872 (+) 2217 WP_406846170.1 RNA ligase -
  WNC56_RS09015 (WNC56_09005) - 1789937..1790647 (+) 711 WP_044335586.1 DUF421 domain-containing protein -
  WNC56_RS09020 (WNC56_09010) - 1790875..1791453 (+) 579 WP_041109214.1 TetR/AcrR family transcriptional regulator -
  WNC56_RS09025 (WNC56_09015) - 1791476..1794619 (+) 3144 WP_151039502.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  WNC56_RS09030 (WNC56_09020) - 1794813..1795721 (+) 909 WP_151039503.1 serine protease -
  WNC56_RS09035 (WNC56_09025) - 1795868..1797874 (+) 2007 WP_151039504.1 glycosyltransferase family 39 protein -
  WNC56_RS09040 (WNC56_09030) nucA/comI 1798100..1798480 (+) 381 WP_044335941.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  WNC56_RS09045 (WNC56_09035) - 1798626..1799474 (+) 849 WP_103132493.1 STAS domain-containing protein -
  WNC56_RS09050 (WNC56_09040) - 1799508..1800050 (-) 543 WP_041109224.1 IseA DL-endopeptidase inhibitor family protein -
  WNC56_RS09055 (WNC56_09045) - 1800345..1800794 (+) 450 WP_073205718.1 hypothetical protein -
  WNC56_RS09060 (WNC56_09050) lexA 1800839..1801459 (-) 621 WP_024425129.1 transcriptional repressor LexA -
  WNC56_RS09065 (WNC56_09055) yneA 1801618..1801929 (+) 312 WP_024425130.1 cell division suppressor protein YneA -
  WNC56_RS09070 (WNC56_09060) - 1801947..1802594 (+) 648 WP_103132492.1 recombinase family protein -
  WNC56_RS09075 (WNC56_09065) - 1802664..1802894 (+) 231 WP_024425132.1 DUF896 domain-containing protein -

Sequence


Protein


Download         Length: 126 a.a.        Molecular weight: 13727.41 Da        Isoelectric Point: 8.5068

>NTDB_id=972779 WNC56_RS09040 WP_044335941.1 1798100..1798480(+) (nucA/comI) [Bacillus safensis strain DMB13]
MGTLLGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=972779 WNC56_RS09040 WP_044335941.1 1798100..1798480(+) (nucA/comI) [Bacillus safensis strain DMB13]
ATGGGCACGTTGTTAGGTGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCAGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTACAATATGTCAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGGCATCAAATCAGCGGGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548


Multiple sequence alignment