Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   WMY94_RS02695 Genome accession   NZ_CP150826
Coordinates   523328..524704 (+) Length   458 a.a.
NCBI ID   WP_023061440.1    Uniprot ID   U4QD78
Organism   Lactobacillus helveticus strain 191404     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 518328..529704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WMY94_RS02670 (WMY94_02670) - 518670..519528 (-) 859 Protein_513 IS982 family transposase -
  WMY94_RS02675 (WMY94_02675) - 519705..520472 (+) 768 WP_023060893.1 IS110 family transposase -
  WMY94_RS02680 (WMY94_02680) - 520937..522161 (-) 1225 Protein_515 RNA-guided endonuclease TnpB family protein -
  WMY94_RS02685 (WMY94_02685) - 522340..522642 (-) 303 WP_003625857.1 hypothetical protein -
  WMY94_RS02690 (WMY94_02690) - 522777..523328 (+) 552 WP_054607197.1 dUTP diphosphatase -
  WMY94_RS02695 (WMY94_02695) radA 523328..524704 (+) 1377 WP_023061440.1 DNA repair protein RadA Machinery gene
  WMY94_RS02700 (WMY94_02700) gltX 524780..526279 (+) 1500 WP_023061441.1 glutamate--tRNA ligase -
  WMY94_RS02705 (WMY94_02705) cysS 526372..527805 (+) 1434 WP_023061442.1 cysteine--tRNA ligase -
  WMY94_RS02710 (WMY94_02710) - 527798..528241 (+) 444 WP_003625863.1 Mini-ribonuclease 3 -
  WMY94_RS02715 (WMY94_02715) rlmB 528228..528980 (+) 753 WP_003625865.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  WMY94_RS02720 (WMY94_02720) - 529123..529668 (+) 546 WP_023061443.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50272.77 Da        Isoelectric Point: 6.5101

>NTDB_id=972695 WMY94_RS02695 WP_023061440.1 523328..524704(+) (radA) [Lactobacillus helveticus strain 191404]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVHLDKIKAEKEERIPTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLAEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDIKPDFVVTDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHTYRILHSVKNRFGAANEIGMFEMENEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMSVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIEARVKEAAKVGFKRIFIPKHNMYQSLTENGIEVIPVASIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=972695 WMY94_RS02695 WP_023061440.1 523328..524704(+) (radA) [Lactobacillus helveticus strain 191404]
ATGGCTAGAGTAAAAACGCAATATAAATGTCGTTCCTGTGGTTATATTTCAGCTAGTTACTTGGGTCGTTGTCCTAACTG
CGGTGCATGGAATCAATTTGAAAAAGAAACTGAAGAAGTACAGAAAAGATCTACTAAAGCAACGGCAAGCCGTTTAATTC
AAAAAACAGGTCTTAATGAACCTGTTCATTTGGATAAAATCAAGGCTGAAAAAGAAGAACGAATTCCTACTAAGTCCGAA
GAATTAAATCGTGTATTAGGTGGCGGGATCGTTCCTGGATCCCTCGTCTTAATTGGTGGGGACCCTGGTATTGGTAAGTC
AACTTTAATGTTGCAGATTATGAGTGATCTGGCAGAAAAATATAAAGTGCTTTATGTTTCAGGAGAAGAGTCAGCTAATC
AAATCAAGCTGAGGGCAGATCGGCTTGGAGTTGGTCAAAGCAACATGCTTTTATATCCAGAAACAGATATGCATGATATT
CGTGAGCAGATCAATGATATTAAGCCTGATTTTGTCGTTACTGATTCTATTCAAACGATGAATGAACCTAGTCTTGATTC
AATGACTGGTTCTGCTTCACAAGTACGTGAGGTAACAAGTGAATTGATGAAGATTGCTAAGATGGATGCAATTACTGTTT
TTGTTATTGGACACGTGACTAAAGAAGGTGCGATTGCTGGCCCCAAAATTTTGGAACACATGGTGGATACTGTCCTTTAT
TTTGAGGGGGATGAACACCACACTTACCGGATTTTGCATTCCGTAAAAAATCGTTTTGGTGCTGCTAACGAAATCGGCAT
GTTTGAAATGGAAAATGAAGGCTTACGTGAAGTAACCAATCCATCTTCGATTTTCCTTGATCAAAGATTACCTAATTCAA
CTGGATCAGCCGTAGTAGTTTCACTTGAAGGAACGCGGCCACTTTTGGCAGAAATTCAAGCATTAGTAACGCCAACTGCA
TTTGGCTATGCCAAACGAACTACTTCAGGAATTAGTTTTAATAAAGCTTCACTATTATTAGCGGTTCTTGAAAAACGCGG
CAATTTAATGCTTCAAAATCAGGATGTTTATTTAACGGCTACTGGCGGTATTAAATTAAATGAACCTGCAATCGATCTAG
CTATTGCTATGTCAGTAGCATCAAGCTACACCGATAAAGAAATCTCACCGACTGATTGTTTTGTAGGGGAAGTGGGATTA
ACCGGTGAAGTTCGCCGCGTTGATAAAATCGAAGCTAGAGTAAAGGAAGCTGCCAAGGTAGGTTTTAAACGTATTTTTAT
TCCTAAGCACAATATGTATCAAAGTCTAACGGAAAATGGCATTGAAGTAATTCCAGTTGCCAGCATTCCACAGGCATTAA
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U4QD78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

56.209

100

0.563

Multiple sequence alignment