Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   WOC64_RS08300 Genome accession   NZ_CP150788
Coordinates   1648159..1649229 (+) Length   356 a.a.
NCBI ID   WP_000775708.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM20816     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1643159..1654229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOC64_RS08270 (WOC64_08275) - 1643582..1645045 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  WOC64_RS08275 (WOC64_08280) - 1645026..1645229 (+) 204 WP_000087561.1 YqgQ family protein -
  WOC64_RS08280 (WOC64_08285) - 1645226..1646212 (+) 987 WP_000161314.1 ROK family glucokinase -
  WOC64_RS08285 (WOC64_08290) - 1646212..1646541 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  WOC64_RS08290 (WOC64_08295) - 1646538..1647161 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  WOC64_RS08295 (WOC64_08300) comGA 1647213..1648187 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  WOC64_RS08300 (WOC64_08305) comGB 1648159..1649229 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  WOC64_RS08305 (WOC64_08310) comGC 1649243..1649554 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  WOC64_RS08310 (WOC64_08315) comGD 1649532..1649978 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  WOC64_RS08315 (WOC64_08320) comGE 1649965..1650264 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  WOC64_RS08320 (WOC64_08325) comGF 1650182..1650679 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  WOC64_RS08325 (WOC64_08330) - 1650776..1650922 (+) 147 WP_001789879.1 hypothetical protein -
  WOC64_RS08330 (WOC64_08335) - 1650912..1651436 (+) 525 WP_001015117.1 shikimate kinase -
  WOC64_RS08335 (WOC64_08340) gcvT 1651595..1652686 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  WOC64_RS08340 (WOC64_08345) gcvPA 1652706..1654052 (+) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41364.77 Da        Isoelectric Point: 10.0092

>NTDB_id=972667 WOC64_RS08300 WP_000775708.1 1648159..1649229(+) (comGB) [Staphylococcus aureus strain TUM20816]
MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=972667 WOC64_RS08300 WP_000775708.1 1648159..1649229(+) (comGB) [Staphylococcus aureus strain TUM20816]
GTGAAACTACAATGCATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.719

100

0.997
  comGB Staphylococcus aureus N315

99.719

100

0.997

Multiple sequence alignment