Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   ACL656_RS03405 Genome accession   NZ_CP178597
Coordinates   709026..709685 (-) Length   219 a.a.
NCBI ID   WP_129949293.1    Uniprot ID   -
Organism   Escherichia coli strain ZF8-1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 704026..714685
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL656_RS03380 (ACL656_03380) yqiA 704094..704675 (+) 582 WP_000105733.1 esterase YqiA -
  ACL656_RS03385 (ACL656_03385) parE 704704..706596 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  ACL656_RS03390 (ACL656_03390) ygiN 706644..706958 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  ACL656_RS03395 (ACL656_03395) mdaB 706989..707570 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  ACL656_RS03400 (ACL656_03400) qseC 707680..709029 (-) 1350 WP_059321712.1 quorum sensing histidine kinase QseC -
  ACL656_RS03405 (ACL656_03405) ciaR 709026..709685 (-) 660 WP_129949293.1 quorum sensing response regulator transcription factor QseB Regulator
  ACL656_RS03410 (ACL656_03410) ygiW 709837..710229 (+) 393 WP_021577970.1 OB fold stress tolerance protein YgiW -
  ACL656_RS03415 (ACL656_03415) ygiV 710282..710764 (+) 483 WP_000183494.1 AraC family transcriptional regulator -
  ACL656_RS03420 (ACL656_03420) ygiS 710873..712480 (+) 1608 WP_129949292.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24814.77 Da        Isoelectric Point: 6.9855

>NTDB_id=972476 ACL656_RS03405 WP_129949293.1 709026..709685(-) (ciaR) [Escherichia coli strain ZF8-1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYRAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=972476 ACL656_RS03405 WP_129949293.1 709026..709685(-) (ciaR) [Escherichia coli strain ZF8-1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATCGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment